Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1838860..1839362 Replicon chromosome
Accession NZ_AP023224
Organism Escherichia coli strain YJ1

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag HXS59_RS08740 Protein ID WP_000767829.1
Coordinates 1839108..1839362 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag HXS59_RS08735 Protein ID WP_001259255.1
Coordinates 1838860..1839111 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HXS59_RS08710 1834037..1835104 - 1068 WP_000080093.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
HXS59_RS08715 1835104..1836174 - 1071 WP_053908449.1 histidinol-phosphate transaminase -
HXS59_RS08720 1836171..1837475 - 1305 WP_053908451.1 histidinol dehydrogenase -
HXS59_RS08725 1837481..1838380 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
HXS59_RS08730 1838526..1838576 - 51 WP_001364200.1 his operon leader peptide -
HXS59_RS08735 1838860..1839111 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
HXS59_RS08740 1839108..1839362 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
HXS59_RS08745 1839445..1840269 + 825 WP_053908453.1 SDR family oxidoreductase -
HXS59_RS08750 1840315..1841244 + 930 WP_053908455.1 LysR family transcriptional regulator -
HXS59_RS08755 1841459..1841521 + 63 WP_010723108.1 membrane protein YoeI -
HXS59_RS08760 1841511..1842869 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
HXS59_RS08765 1843048..1844106 + 1059 WP_053908457.1 transport protein YeeE -
HXS59_RS08770 1844120..1844347 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T36930 WP_000767829.1 NZ_AP023224:1839108-1839362 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T36930 NZ_AP023224:1839108-1839362 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAGTCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAGCATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT36930 WP_001259255.1 NZ_AP023224:1838860-1839111 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT36930 NZ_AP023224:1838860-1839111 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References