Detailed information of TA system TA036917

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 123921..124175
• Replicon: plasmid pM505-a
• Accession: NZ_AP023221
• Organism: Escherichia coli strain M505

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: EPG46_RS24450
• Protein ID: WP_032215358.1
• Coordinates: 123921..124127 (-)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	124114..124175 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EPG46_RS24410	119302..119717	-	416	Protein_130	IS1 family transposase	-
EPG46_RS24415	119966..120340	-	375	WP_176488922.1	type II toxin-antitoxin system PemK/MazF family toxin	-
EPG46_RS24420	120301..120558	-	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
EPG46_RS24425	120651..120985	-	335	Protein_133	CPBP family intramembrane metalloprotease	-
EPG46_RS24430	121408..121548	-	141	WP_001333237.1	hypothetical protein	-
EPG46_RS24435	122249..123077	-	829	Protein_135	incFII family plasmid replication initiator RepA	-
EPG46_RS24440	123070..123144	-	75	WP_001365705.1	RepA leader peptide Tap	-
EPG46_RS24445	123380..123637	-	258	WP_000083833.1	replication regulatory protein RepA	-
EPG46_RS24450	123921..124127	-	207	WP_032215358.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	124114..124175	+	62	NuclAT_0	-	Antitoxin
-	124114..124175	+	62	NuclAT_0	-	Antitoxin
-	124114..124175	+	62	NuclAT_0	-	Antitoxin
-	124114..124175	+	62	NuclAT_0	-	Antitoxin
EPG46_RS24455	124314..124580	-	267	WP_087757657.1	hypothetical protein	-
EPG46_RS24460	124955..125524	-	570	WP_029702150.1	DUF2726 domain-containing protein	-
EPG46_RS24465	125676..126302	-	627	WP_029702149.1	hypothetical protein	-
EPG46_RS24470	126714..126923	-	210	WP_039023209.1	hypothetical protein	-
EPG46_RS24475	127054..127614	-	561	WP_001567328.1	fertility inhibition protein FinO	-
EPG46_RS24480	127669..128415	-	747	WP_052273601.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(B) / blaTEM-1B / blaCTX-M-15 / qnrS1	-	1..130968	130968

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7778.27 Da
• Isoelectric Point: 8.8807

>T36917 WP_032215358.1 NZ_AP023221:c124127-123921 [Escherichia coli]
MKYLNTTDCSLFLAERSKFMTKYALIGVLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T36917 NZ_AP023221:c124127-123921 [Escherichia coli]
ATGAAGTACCTTAACACTACTGATTGTAGCCTCTTCCTTGCAGAGAGGTCAAAGTTTATGACGAAATATGCCCTTATCGG
GGTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTTATGTGAACTGAATATTCACAGGG
GAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT36917 NZ_AP023221:124114-124175 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin