Detailed information of TA system TA036910

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 4997..5423
• Replicon: plasmid pM505-NDM5
• Accession: NZ_AP023220
• Organism: Escherichia coli strain M505

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: EPG46_RS23190
• Protein ID: WP_001312861.1
• Coordinates: 4997..5155 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	5199..5423 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EPG46_RS23155	20..247	-	228	WP_000089263.1	conjugal transfer relaxosome protein TraY	-
EPG46_RS23160	384..1055	-	672	WP_000283561.1	conjugal transfer transcriptional regulator TraJ	-
EPG46_RS23165	1249..1632	-	384	WP_001354030.1	relaxosome protein TraM	-
EPG46_RS23170	1955..2557	+	603	WP_000243713.1	transglycosylase SLT domain-containing protein	-
EPG46_RS23175	2854..3675	-	822	WP_001234445.1	DUF945 domain-containing protein	-
EPG46_RS23180	3786..4082	-	297	WP_001272251.1	hypothetical protein	-
EPG46_RS23185	4382..4759	+	378	Protein_6	hypothetical protein	-
EPG46_RS23190	4997..5155	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	5199..5423	-	225	NuclAT_0	-	Antitoxin
-	5199..5423	-	225	NuclAT_0	-	Antitoxin
-	5199..5423	-	225	NuclAT_0	-	Antitoxin
-	5199..5423	-	225	NuclAT_0	-	Antitoxin
EPG46_RS23195	5235..5414	+	180	WP_001309233.1	hypothetical protein	-
EPG46_RS23200	5435..6154	-	720	WP_001276217.1	plasmid SOS inhibition protein A	-
EPG46_RS23205	6151..6585	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
EPG46_RS23210	6654..8677	-	2024	Protein_11	ParB/RepB/Spo0J family partition protein	-
EPG46_RS23215	8738..8971	-	234	WP_000006003.1	DUF905 family protein	-
EPG46_RS23220	9029..9556	-	528	WP_000290834.1	single-stranded DNA-binding protein	-
EPG46_RS23225	9865..10327	+	463	Protein_14	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	dfrA12 / aadA2 / qacE / sul1 / blaNDM-5 / rmtB / blaTEM-1B / mph(A) / erm(B)	-	1..97234	97234

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T36910 WP_001312861.1 NZ_AP023220:c5155-4997 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T36910 NZ_AP023220:c5155-4997 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT36910 NZ_AP023220:c5423-5199 [Escherichia coli]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin