Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 61118..61812 Replicon chromosome
Accession NZ_AP023219
Organism Escherichia coli strain M505

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag EPG46_RS00295 Protein ID WP_001263489.1
Coordinates 61414..61812 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag EPG46_RS00290 Protein ID WP_000554758.1
Coordinates 61118..61411 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EPG46_RS00270 56750..57247 + 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
EPG46_RS00275 57471..59183 - 1713 Protein_47 flagellar biosynthesis protein FlhA -
EPG46_RS00280 59155..59940 + 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
EPG46_RS00285 60011..61066 + 1056 WP_001226164.1 DNA polymerase IV -
EPG46_RS00290 61118..61411 + 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
EPG46_RS00295 61414..61812 + 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
EPG46_RS00300 61822..62274 + 453 WP_001059892.1 GNAT family N-acetyltransferase -
EPG46_RS00305 62592..62798 + 207 Protein_53 RtcB family protein -
EPG46_RS00310 62794..63315 + 522 Protein_54 peptide chain release factor H -
EPG46_RS00315 63372..64829 - 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
EPG46_RS00320 65090..65548 + 459 WP_001291990.1 xanthine phosphoribosyltransferase -
- 66144..66224 + 81 NuclAT_12 - -
- 66144..66224 + 81 NuclAT_12 - -
- 66144..66224 + 81 NuclAT_12 - -
- 66144..66224 + 81 NuclAT_12 - -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island catA1 / qepA1 / blaOXA-1 / ant(3'')-Ia gmhA/lpcA 47355..96709 49354


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T36888 WP_001263489.1 NZ_AP023219:61414-61812 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T36888 NZ_AP023219:61414-61812 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT36888 WP_000554758.1 NZ_AP023219:61118-61411 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT36888 NZ_AP023219:61118-61411 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References