36881

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 388348..388889 Replicon plasmid pMTY18781-1_lncX3
Accession NZ_AP023206
Organism Escherichia coli strain TUM18781

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag HUV20_RS21390 Protein ID WP_000615976.1
Coordinates 388348..388626 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag HUV20_RS21395 Protein ID WP_000729704.1
Coordinates 388629..388889 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUV20_RS21375 385930..386508 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
HUV20_RS21380 386714..387481 + 768 WP_000333380.1 class II glutamine amidotransferase -
HUV20_RS21385 387452..388192 - 741 WP_001225679.1 murein L,D-transpeptidase -
HUV20_RS21390 388348..388626 - 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
HUV20_RS21395 388629..388889 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
HUV20_RS21400 389075..389848 + 774 WP_000543897.1 C40 family peptidase -
HUV20_RS21405 389905..390261 - 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
HUV20_RS21410 390254..390532 - 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
HUV20_RS21415 390637..392730 - 2094 WP_089586219.1 flagellar biosynthesis protein FlhA -
HUV20_RS21420 392714..393853 - 1140 WP_053264702.1 flagellar type III secretion system protein FlhB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - fimA / fimI / fimC / fimD / fimF / fimG / fimH / cheD / espX1 / lpxC / lpxD / lpxA / IlpA / hcp1/tssD1 / tssA / tssA / clpV/tssH / tssL / tssJ / fha / tssG / tssF / AHA_1833 / tssB / hcp2/tssD2 / vgrG/tssI / rhs/PAAR / rhs/PAAR / rhs/PAAR / gmhA/lpcA 1..404189 404189


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T36881 WP_000615976.1 NZ_AP023206:c388626-388348 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T36881 NZ_AP023206:c388626-388348 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT36881 WP_000729704.1 NZ_AP023206:c388889-388629 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT36881 NZ_AP023206:c388889-388629 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References