Detailed information of TA system TA036857

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 89624..90050
• Replicon: plasmid pMTY18780-2
• Accession: NZ_AP023199
• Organism: Escherichia coli strain TUM18780

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: HUV22_RS25020
• Protein ID: WP_001312861.1
• Coordinates: 89624..89782 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	89826..90050 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUV22_RS24990	84998..85687	-	690	WP_000283385.1	conjugal transfer transcriptional regulator TraJ	-
HUV22_RS24995	85874..86257	-	384	WP_001151524.1	relaxosome protein TraM	-
HUV22_RS25000	86578..87180	+	603	WP_174402700.1	transglycosylase SLT domain-containing protein	-
HUV22_RS25005	87477..88298	-	822	WP_001234426.1	DUF945 domain-containing protein	-
HUV22_RS25010	88416..88703	-	288	WP_000107535.1	hypothetical protein	-
HUV22_RS25020	89624..89782	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	89826..90050	-	225	NuclAT_0	-	Antitoxin
-	89826..90050	-	225	NuclAT_0	-	Antitoxin
-	89826..90050	-	225	NuclAT_0	-	Antitoxin
-	89826..90050	-	225	NuclAT_0	-	Antitoxin
HUV22_RS25025	89862..90050	+	189	WP_001299721.1	hypothetical protein	-
HUV22_RS25030	90062..90781	-	720	WP_001276238.1	plasmid SOS inhibition protein A	-
HUV22_RS25035	90778..91212	-	435	WP_000845936.1	conjugation system SOS inhibitor PsiB	-
HUV22_RS25040	91267..91464	-	198	Protein_110	hypothetical protein	-
HUV22_RS25045	91492..91725	-	234	WP_000005987.1	DUF905 family protein	-
HUV22_RS25050	91793..92332	-	540	WP_000290840.1	single-stranded DNA-binding protein	-
HUV22_RS25055	92358..92564	-	207	WP_000275856.1	hypothetical protein	-
HUV22_RS25065	93660..94631	-	972	WP_000817028.1	ParB/RepB/Spo0J family plasmid partition protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(A) / sitABCD	iutA / iucD / iucC / iucB / iucB / iucA	1..115013	115013

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T36857 WP_001312861.1 NZ_AP023199:c89782-89624 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T36857 NZ_AP023199:c89782-89624 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT36857 NZ_AP023199:c90050-89826 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin