Detailed information of TA system TA036852

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 51295..51549
• Replicon: plasmid pMTY18780-2
• Accession: NZ_AP023199
• Organism: Escherichia coli strain TUM18780

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: HUV22_RS24795
• Protein ID: WP_001351576.1
• Coordinates: 51295..51501 (-)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	51488..51549 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUV22_RS24755	46847..47248	-	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
HUV22_RS24760	47181..47438	-	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
HUV22_RS24765	47531..48184	-	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
HUV22_RS24770	48282..48422	-	141	WP_001333237.1	hypothetical protein	-
HUV22_RS24775	49123..49980	-	858	WP_000774297.1	incFII family plasmid replication initiator RepA	-
HUV22_RS24780	49973..50455	-	483	WP_001273588.1	hypothetical protein	-
HUV22_RS24785	50448..50522	-	75	WP_001442103.1	RepA leader peptide Tap	-
HUV22_RS24790	50754..51011	-	258	WP_000083845.1	replication regulatory protein RepA	-
HUV22_RS24795	51295..51501	-	207	WP_001351576.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	51488..51549	+	62	NuclAT_1	-	Antitoxin
-	51488..51549	+	62	NuclAT_1	-	Antitoxin
-	51488..51549	+	62	NuclAT_1	-	Antitoxin
-	51488..51549	+	62	NuclAT_1	-	Antitoxin
HUV22_RS24800	51805..51879	-	75	Protein_63	endonuclease	-
HUV22_RS24805	52125..52337	-	213	WP_001299730.1	ANR family transcriptional regulator	-
HUV22_RS24810	52473..53033	-	561	WP_000139315.1	fertility inhibition protein FinO	-
HUV22_RS24815	53136..53996	-	861	WP_000704513.1	alpha/beta hydrolase	-
HUV22_RS24820	54055..54801	-	747	WP_000205749.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(A) / sitABCD	iutA / iucD / iucC / iucB / iucB / iucA	1..115013	115013

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7826.31 Da
• Isoelectric Point: 8.8807

>T36852 WP_001351576.1 NZ_AP023199:c51501-51295 [Escherichia coli]
MKYLNTTDCSLFFAERSKFMTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T36852 NZ_AP023199:c51501-51295 [Escherichia coli]
ATGAAGTACCTTAACACTACTGATTGTAGCCTCTTCTTTGCTGAGAGGTCAAAGTTTATGACGAAATATGCCCTTATCGG
GTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTTATGTGAGCTGAATATTCACAGAG
GAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT36852 NZ_AP023199:51488-51549 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin