Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3840850..3841391 Replicon chromosome
Accession NZ_AP023197
Organism Escherichia coli strain TUM18780

Toxin (Protein)


Gene name yafQ Uniprot ID Q32JN3
Locus tag HUV22_RS18160 Protein ID WP_000615980.1
Coordinates 3841113..3841391 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q32JN2
Locus tag HUV22_RS18155 Protein ID WP_000729709.1
Coordinates 3840850..3841110 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUV22_RS18140 3836932..3839025 + 2094 WP_021556448.1 flagellar biosynthesis protein FlhA -
HUV22_RS18145 3839218..3839715 - 498 WP_021556447.1 REP-associated tyrosine transposase RayT -
HUV22_RS18150 3839891..3840640 - 750 WP_000056850.1 C40 family peptidase -
HUV22_RS18155 3840850..3841110 + 261 WP_000729709.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
HUV22_RS18160 3841113..3841391 + 279 WP_000615980.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
HUV22_RS18165 3841547..3842287 + 741 WP_001225679.1 murein L,D-transpeptidase -
HUV22_RS18170 3842258..3843025 - 768 WP_000333379.1 class II glutamine amidotransferase -
HUV22_RS18175 3843231..3843809 - 579 WP_089628370.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 3839891..3849643 9752


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(5-92)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10830.62 Da        Isoelectric Point: 10.0702

>T36844 WP_000615980.1 NZ_AP023197:3841113-3841391 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWLLIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T36844 NZ_AP023197:3841113-3841391 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGCTCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9333.73 Da        Isoelectric Point: 5.8129

>AT36844 WP_000729709.1 NZ_AP023197:3840850-3841110 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVGVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT36844 NZ_AP023197:3840850-3841110 [Escherichia coli]
ATGGCTGCAAATGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGGTGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090NH48

Antitoxin

Source ID Structure
AlphaFold DB A0A829FY17

References