Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1821546..1822048 Replicon chromosome
Accession NZ_AP023197
Organism Escherichia coli strain TUM18780

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag HUV22_RS08505 Protein ID WP_000767829.1
Coordinates 1821794..1822048 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag HUV22_RS08500 Protein ID WP_001259255.1
Coordinates 1821546..1821797 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HUV22_RS08475 1816725..1817792 - 1068 WP_000080085.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
HUV22_RS08480 1817792..1818862 - 1071 WP_000108940.1 histidinol-phosphate transaminase -
HUV22_RS08485 1818859..1820163 - 1305 WP_001515511.1 histidinol dehydrogenase -
HUV22_RS08490 1820169..1821068 - 900 WP_174402630.1 ATP phosphoribosyltransferase -
HUV22_RS08495 1821214..1821264 - 51 WP_001364200.1 his operon leader peptide -
HUV22_RS08500 1821546..1821797 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
HUV22_RS08505 1821794..1822048 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
HUV22_RS08510 1822131..1822955 + 825 WP_000754763.1 SDR family oxidoreductase -
HUV22_RS08515 1823001..1823930 + 930 WP_000803351.1 LysR family transcriptional regulator -
HUV22_RS08520 1824145..1824207 + 63 WP_010723108.1 membrane protein YoeI -
HUV22_RS08525 1824197..1825555 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
HUV22_RS08530 1825734..1826792 + 1059 WP_023568741.1 transport protein YeeE -
HUV22_RS08535 1826806..1827033 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T36831 WP_000767829.1 NZ_AP023197:1821794-1822048 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T36831 NZ_AP023197:1821794-1822048 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT36831 WP_001259255.1 NZ_AP023197:1821546-1821797 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT36831 NZ_AP023197:1821546-1821797 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References