Detailed information of TA system TA036823

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 90111..90537
• Replicon: plasmid pMTY18530-2
• Accession: NZ_AP023192
• Organism: Escherichia coli strain TUM18530

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: HUV24_RS25010
• Protein ID: WP_001312861.1
• Coordinates: 90111..90269 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	90313..90537 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HUV24_RS24980	85485..86174	-	690	WP_000283385.1	conjugal transfer transcriptional regulator TraJ	-
HUV24_RS24985	86361..86744	-	384	WP_001151524.1	relaxosome protein TraM	-
HUV24_RS24990	87065..87667	+	603	WP_174402700.1	transglycosylase SLT domain-containing protein	-
HUV24_RS24995	87964..88785	-	822	WP_001234426.1	DUF945 domain-containing protein	-
HUV24_RS25000	88903..89190	-	288	WP_000107535.1	hypothetical protein	-
HUV24_RS25010	90111..90269	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	90313..90537	-	225	NuclAT_0	-	Antitoxin
-	90313..90537	-	225	NuclAT_0	-	Antitoxin
-	90313..90537	-	225	NuclAT_0	-	Antitoxin
-	90313..90537	-	225	NuclAT_0	-	Antitoxin
HUV24_RS25015	90349..90537	+	189	WP_001299721.1	hypothetical protein	-
HUV24_RS25020	90549..91268	-	720	WP_001276238.1	plasmid SOS inhibition protein A	-
HUV24_RS25025	91265..91699	-	435	WP_000845936.1	conjugation system SOS inhibitor PsiB	-
HUV24_RS25030	91754..91951	-	198	Protein_108	hypothetical protein	-
HUV24_RS25035	91979..92212	-	234	WP_000005987.1	DUF905 family protein	-
HUV24_RS25040	92280..92819	-	540	WP_000290840.1	single-stranded DNA-binding protein	-
HUV24_RS25045	92845..93051	-	207	WP_000275856.1	hypothetical protein	-
HUV24_RS25055	94190..95161	-	972	WP_000817028.1	ParB/RepB/Spo0J family plasmid partition protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(A) / blaCTX-M-55 / sitABCD	iutA / iucD / iucC / iucB / iucB / iucA	1..115543	115543

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T36823 WP_001312861.1 NZ_AP023192:c90269-90111 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T36823 NZ_AP023192:c90269-90111 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT36823 NZ_AP023192:c90537-90313 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin