Detailed information of TA system TA036769

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 1589001..1589667
• Replicon: chromosome
• Accession: NZ_AP023184
• Organism: Buttiauxella agrestis strain DSM 9389

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: BADSM9389_RS07370
• Protein ID: WP_172893699.1
• Coordinates: 1589001..1589348 (+)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: BADSM9389_RS07375
• Protein ID: WP_172893700.1
• Coordinates: 1589350..1589667 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BADSM9389_RS07360	1584393..1587524	-	3132	WP_172893698.1	MdtB/MuxB family multidrug efflux RND transporter permease subunit	-
BADSM9389_RS07365	1587524..1588771	-	1248	WP_172896099.1	MdtA/MuxA family multidrug efflux RND transporter periplasmic adaptor subunit	-
BADSM9389_RS07370	1589001..1589348	+	348	WP_172893699.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BADSM9389_RS07375	1589350..1589667	+	318	WP_172893700.1	helix-turn-helix transcriptional regulator	Antitoxin
BADSM9389_RS07380	1589698..1591056	-	1359	WP_172893701.1	molecular chaperone	-
BADSM9389_RS07385	1591190..1592035	+	846	WP_172893702.1	DNA-3-methyladenine glycosylase 2	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (39-87)

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 13738.00 Da
• Isoelectric Point: 10.3593

>T36769 WP_172893699.1 NZ_AP023184:1589001-1589348 [Buttiauxella agrestis]
MTWKIVTTKRFEEWFDQQSNVLQERMLASLVRLHFLGPGLPRPFADTIKGSHFTNMKELRVQFQGKPIRAFYIFDFQRNA
ILLCAGDKSKSKRFYETMVNVADKEFMDWLRLQEK

Nucleotide

• Length: 348 bp

>T36769 NZ_AP023184:1589001-1589348 [Buttiauxella agrestis]
ATGACATGGAAGATAGTGACGACAAAACGGTTTGAAGAGTGGTTTGATCAGCAAAGTAATGTACTTCAGGAAAGGATGCT
TGCGAGTTTGGTGCGGTTACATTTTTTGGGACCTGGATTACCTCGCCCTTTTGCGGACACCATAAAGGGATCGCATTTTA
CCAATATGAAAGAACTTAGAGTTCAATTTCAAGGTAAGCCCATCAGAGCTTTTTATATTTTTGATTTTCAGCGTAATGCG
ATTTTGTTGTGTGCCGGGGATAAGTCGAAAAGTAAGCGTTTTTACGAAACGATGGTTAATGTAGCAGACAAAGAGTTTAT
GGACTGGTTGAGGTTACAGGAGAAATAA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11907.86 Da
• Isoelectric Point: 7.1362

>AT36769 WP_172893700.1 NZ_AP023184:1589350-1589667 [Buttiauxella agrestis]
MKKLPEGYTTLEELIARRSPESQKRIAEETQELILECCLHMMREQKGWSQQQLAEVMGISQPAVTAIEKRGNEVKLGTLK
RYIEALGGKLSLHIEFPDGVKQLSI

Nucleotide

• Length: 318 bp

>AT36769 NZ_AP023184:1589350-1589667 [Buttiauxella agrestis]
ATGAAAAAATTACCCGAGGGTTACACCACATTAGAAGAGCTTATCGCCCGACGCTCTCCAGAGAGCCAGAAGCGAATTGC
CGAAGAAACTCAGGAGCTGATTCTCGAATGCTGTCTGCATATGATGCGGGAGCAAAAAGGCTGGTCGCAGCAGCAACTGG
CCGAAGTCATGGGTATTAGCCAACCCGCCGTCACTGCAATTGAAAAGCGCGGTAATGAAGTCAAACTAGGGACGCTAAAG
CGCTATATCGAGGCGTTGGGCGGCAAGCTTTCGCTGCACATAGAGTTCCCGGACGGGGTGAAACAACTCAGTATTTAA