Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 358541..359187 Replicon chromosome
Accession NZ_AP023148
Organism Klebsiella pneumoniae strain SMKP03

Toxin (Protein)


Gene name higB Uniprot ID A0A231WWF9
Locus tag SMKP03_RS01655 Protein ID WP_004174016.1
Coordinates 358541..358888 (+) Length 116 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A4S7L580
Locus tag SMKP03_RS01660 Protein ID WP_019725405.1
Coordinates 358888..359187 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SMKP03_RS01645 354467..355900 + 1434 WP_002920564.1 glycogen synthase GlgA -
SMKP03_RS01650 355918..358365 + 2448 WP_121593178.1 glycogen phosphorylase -
SMKP03_RS01655 358541..358888 + 348 WP_004174016.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
SMKP03_RS01660 358888..359187 + 300 WP_019725405.1 XRE family transcriptional regulator Antitoxin
SMKP03_RS01665 359250..360758 - 1509 WP_002920554.1 glycerol-3-phosphate dehydrogenase -
SMKP03_RS01670 360963..361292 + 330 WP_002920552.1 thiosulfate sulfurtransferase GlpE -
SMKP03_RS01675 361343..362173 + 831 WP_004151408.1 rhomboid family intramembrane serine protease GlpG -
SMKP03_RS01680 362223..362981 + 759 WP_002920548.1 DeoR/GlpR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(13-107)

Antitoxin

Cro/C1-type helix-turn-helix domain

(31-84)


Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 13518.55 Da        Isoelectric Point: 6.2327

>T36740 WP_004174016.1 NZ_AP023148:358541-358888 [Klebsiella pneumoniae]
MWDVETTDTFDAWFELQSRALKEDMLATMLILSEFAPQLGRPYVDTVKDSTFQNMKELRVQHHGLPIRAFFAFDPLRKAI
VLCAGNKDGMNEKRFYKEMITLADREFSQHLTKER

Download         Length: 348 bp

>T36740 NZ_AP023148:358541-358888 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATGCCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTCCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTTTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGCGGGCAATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10775.53 Da        Isoelectric Point: 9.7681

>AT36740 WP_019725405.1 NZ_AP023148:358888-359187 [Klebsiella pneumoniae]
MATLKELMAKQSPESQQRIAAKAAEIRQSVALNLLREELQMSQTEMAAAMGVKQPTIAKMEQTDNDPRLSTLKSYIAALG
GELSINVTLPTGKKVSFNL

Download         Length: 300 bp

>AT36740 NZ_AP023148:358888-359187 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGAAAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGACTGATAACGACCCTCGGCTTTCGACCCTAAAAAGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A231WWF9

Antitoxin

Source ID Structure
AlphaFold DB A0A4S7L580

References