Detailed information of TA system TA036723

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 5556..6214
• Replicon: plasmid pOCU_Ac16a_1
• Accession: NZ_AP023078
• Organism: Acinetobacter baumannii strain OCU_Ac16a

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: OCUAC_RS19150
• Protein ID: WP_000312250.1
• Coordinates: 5855..6214 (-)
• Length: 120 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: OCUAC_RS19145
• Protein ID: WP_001096429.1
• Coordinates: 5556..5855 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OCUAC_RS19115	970..2226	+	1257	WP_072689898.1	ParB/RepB/Spo0J family partition protein	-
OCUAC_RS19120	2362..3591	+	1230	WP_033854393.1	hypothetical protein	-
OCUAC_RS19125	3606..3839	+	234	WP_033854394.1	hypothetical protein	-
OCUAC_RS19130	3880..4137	+	258	WP_000834290.1	hypothetical protein	-
OCUAC_RS19135	4142..4714	+	573	WP_000443897.1	hypothetical protein	-
OCUAC_RS19140	4690..4869	+	180	WP_033854395.1	hypothetical protein	-
OCUAC_RS19145	5556..5855	-	300	WP_001096429.1	helix-turn-helix transcriptional regulator	Antitoxin
OCUAC_RS19150	5855..6214	-	360	WP_000312250.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
OCUAC_RS19155	6415..6981	+	567	WP_000710385.1	hypothetical protein	-
OCUAC_RS19160	7188..8537	-	1350	WP_072689902.1	ISL3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..73028	73028

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (14-109)

Antitoxin

• HigA2-like, helix-turn-helix domain (12-88)

Sequences

Toxin

Protein

• Length: 120 a.a.
• Molecular weight: 13751.62 Da
• Isoelectric Point: 4.8212

>T36723 WP_000312250.1 NZ_AP023078:c6214-5855 [Acinetobacter baumannii]
MAWDVETTELFDSWLAEQDENAQDKILASLLVLSELGPNLGRPHVDTIKESKYPNMKEIRVQVKGHPIRGFFAFDPERKA
IVLCAGDKKGLNEKAFYKEMIKIADEQYEQYLRDNYGDK

Nucleotide

• Length: 360 bp

>T36723 NZ_AP023078:c6214-5855 [Acinetobacter baumannii]
ATGGCTTGGGATGTAGAAACAACAGAACTTTTTGATTCTTGGTTGGCAGAACAAGATGAAAATGCTCAAGATAAAATCTT
AGCGTCTTTGCTTGTTTTGTCAGAACTGGGGCCAAATCTAGGCCGGCCTCATGTTGATACTATTAAAGAATCAAAATATC
CGAACATGAAAGAGATACGTGTGCAAGTTAAAGGTCATCCGATCCGTGGTTTCTTTGCATTTGATCCAGAACGTAAAGCC
ATTGTTTTGTGTGCTGGAGATAAGAAAGGGCTAAATGAAAAAGCCTTTTATAAAGAAATGATCAAGATTGCAGATGAACA
ATATGAGCAATATTTACGTGATAATTACGGAGATAAATAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11012.93 Da
• Isoelectric Point: 10.4856

>AT36723 WP_001096429.1 NZ_AP023078:c5855-5556 [Acinetobacter baumannii]
MAKLNDIISKRSPESQQRIKKMANQMLLDINLSNLREELKISQAEIAKNLGVSQAAISKREKLGKDLKISSLKEYVEAMG
GKVRLDVELPNGKHVAISI

Nucleotide

• Length: 300 bp

>AT36723 NZ_AP023078:c5855-5556 [Acinetobacter baumannii]
ATGGCTAAATTAAACGATATAATTTCAAAACGCTCACCTGAAAGCCAACAACGCATCAAGAAAATGGCAAATCAAATGCT
ATTGGATATTAACCTATCGAACCTACGGGAAGAGTTAAAAATATCTCAAGCTGAAATTGCAAAAAATCTAGGTGTAAGCC
AAGCTGCTATTTCAAAACGTGAAAAATTAGGTAAAGATCTTAAGATTTCATCATTAAAAGAATATGTTGAAGCTATGGGT
GGCAAAGTAAGGTTAGATGTTGAGTTACCTAACGGAAAGCATGTTGCTATATCTATTTAA