Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3390690..3391266 Replicon chromosome
Accession NZ_AP022869
Organism Halomonas meridiana strain Eplume2

Toxin (Protein)


Gene name higB Uniprot ID G6YV50
Locus tag HMEPL2_RS16055 Protein ID WP_008174254.1
Coordinates 3390690..3390968 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name graA Uniprot ID A0A1I1JKC7
Locus tag HMEPL2_RS16060 Protein ID WP_036148342.1
Coordinates 3390979..3391266 (+) Length 96 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HMEPL2_RS16030 3386622..3387251 + 630 WP_172515445.1 hypothetical protein -
HMEPL2_RS16035 3387296..3388525 + 1230 WP_172515738.1 hypothetical protein -
HMEPL2_RS16040 3388522..3389388 + 867 WP_172515446.1 hypothetical protein -
HMEPL2_RS16045 3389637..3389942 + 306 WP_172515447.1 hypothetical protein -
HMEPL2_RS16050 3390028..3390573 + 546 WP_172515448.1 GNAT family N-acetyltransferase -
HMEPL2_RS16055 3390690..3390968 + 279 WP_008174254.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
HMEPL2_RS16060 3390979..3391266 + 288 WP_036148342.1 HigA family addiction module antidote protein Antitoxin
HMEPL2_RS16065 3391660..3393015 - 1356 WP_172515449.1 IS1182 family transposase -
HMEPL2_RS16070 3393060..3393275 + 216 WP_172515450.1 type II toxin-antitoxin system RelE/ParE family toxin -
HMEPL2_RS16075 3393290..3393604 + 315 WP_008174257.1 HigA family addiction module antidote protein -
HMEPL2_RS16080 3393679..3393951 - 273 WP_054641814.1 BrnA antitoxin family protein -
HMEPL2_RS16085 3393926..3394237 - 312 WP_054641813.1 BrnT family toxin -
HMEPL2_RS16090 3394342..3394671 + 330 WP_197746083.1 hypothetical protein -
HMEPL2_RS16095 3394850..3395107 + 258 WP_022992576.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
HMEPL2_RS16100 3395095..3395397 + 303 WP_054641811.1 type II toxin-antitoxin system RelE/ParE family toxin -
HMEPL2_RS16105 3395534..3395803 + 270 WP_022990375.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
HMEPL2_RS16110 3395804..3396103 + 300 WP_062373125.1 type II toxin-antitoxin system RelE/ParE family toxin -
HMEPL2_RS16115 3396015..3396230 + 216 WP_084812740.1 type II toxin-antitoxin system CcdA family antitoxin -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 3387248..3403389 16141
- flank IS/Tn - - 3391660..3393015 1355


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(4-92)

Antitoxin

(3-83)


Sequences


Toxin        


Download         Length: 93 a.a.        Molecular weight: 10345.76 Da        Isoelectric Point: 10.3742

>T36613 WP_008174254.1 NZ_AP022869:3390690-3390968 [Halomonas meridiana]
MIRSFKHKGLAKFFKSGSTAGIQAAHEKRLRLILGRLNAASNAKDMDLPGLRLHELSGNRAGIWSVTVSGNWRVTFRFED
GDAEIVNYEDYH

Download         Length: 279 bp

>T36613 NZ_AP022869:3390690-3390968 [Halomonas meridiana]
ATGATTCGAAGCTTCAAACACAAGGGCTTGGCAAAATTCTTCAAGTCCGGCAGTACTGCAGGGATTCAGGCCGCTCATGA
AAAGAGGCTTCGGCTAATTCTCGGTAGATTGAATGCAGCATCGAATGCCAAAGATATGGACTTACCCGGTCTTCGCTTGC
ATGAGCTCTCTGGCAATCGTGCCGGAATCTGGTCAGTGACCGTCAGCGGTAACTGGCGAGTGACCTTCCGGTTCGAAGAC
GGAGATGCCGAGATTGTGAACTACGAAGATTATCACTGA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10400.00 Da        Isoelectric Point: 6.8695

>AT36613 WP_036148342.1 NZ_AP022869:3390979-3391266 [Halomonas meridiana]
MLMHNPPHPGEVLRELCLEPLGLSVTAAAEALGVSRKTLSAVLNGKAGISPEMAIRLSIAFDTSAESWLNQQSQYELWHA
EQHRKELNVKKLVAA

Download         Length: 288 bp

>AT36613 NZ_AP022869:3390979-3391266 [Halomonas meridiana]
ATGTTAATGCACAACCCTCCGCACCCGGGTGAAGTGCTGCGAGAGCTTTGTCTTGAGCCCCTTGGCCTTTCTGTCACAGC
AGCAGCAGAGGCATTGGGCGTTAGCCGCAAAACGCTGAGTGCCGTGTTGAATGGCAAGGCCGGCATTAGTCCAGAAATGG
CGATCCGGCTCTCTATCGCTTTCGATACCTCGGCAGAAAGCTGGCTAAATCAGCAGTCCCAATATGAACTTTGGCATGCT
GAACAACATCGCAAAGAGCTCAACGTGAAGAAGCTTGTTGCAGCCTGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB G6YV50


Antitoxin

Source ID Structure
AlphaFold DB A0A1I1JKC7

References