Detailed information of TA system TA036602

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1331043..1331682
• Replicon: chromosome
• Accession: NZ_AP022867
• Organism: Haemophilus influenzae strain 2018-Y40

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q4QJX7
• Locus tag: HIY_RS06705
• Protein ID: WP_005650215.1
• Coordinates: 1331043..1331348 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HIY_RS06710
• Protein ID: WP_005650217.1
• Coordinates: 1331359..1331682 (+)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HIY_RS06685	1326472..1328511	-	2040	WP_005646675.1	excinuclease ABC subunit B	-
HIY_RS06695	1329127..1330095	-	969	WP_005657582.1	zinc transporter permease subunit ZevB	-
HIY_RS06700	1330098..1330718	-	621	WP_005657580.1	zinc transporter binding subunit ZevA	-
HIY_RS06705	1331043..1331348	+	306	WP_005650215.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HIY_RS06710	1331359..1331682	+	324	WP_005650217.1	HigA family addiction module antidote protein	Antitoxin
HIY_RS06715	1331845..1333515	+	1671	WP_005657578.1	energy-dependent translational throttle protein EttA	-
HIY_RS06720	1333577..1333918	+	342	WP_005657576.1	SirB2 family protein	-
HIY_RS06725	1333923..1335893	+	1971	WP_005657574.1	tRNA(Met) cytidine acetyltransferase TmcA	-
HIY_RS06730	1335890..1336063	+	174	WP_005657572.1	DUF5363 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-99)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (4-82)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 12301.93 Da
• Isoelectric Point: 9.0853

>T36602 WP_005650215.1 NZ_AP022867:1331043-1331348 [Haemophilus influenzae]
MFNLKREHFRDDYLYRFYQYGDTHSKIPSNLYKVLARKLDMISASENINDLRSPPANHLELLEPKENKIYSIRVNKQYRL
IFKYENNEVNNLYLDPHSYNL

Nucleotide

• Length: 306 bp

>T36602 NZ_AP022867:1331043-1331348 [Haemophilus influenzae]
ATGTTTAATTTAAAGCGTGAGCATTTTAGAGATGATTATCTTTATAGGTTTTATCAATATGGTGATACTCATAGTAAGAT
TCCTTCTAATTTGTATAAAGTATTGGCAAGAAAATTAGATATGATAAGTGCCTCAGAAAATATCAACGATTTACGTTCTC
CACCAGCTAACCATTTGGAGTTGCTTGAGCCAAAAGAAAATAAAATTTATTCCATTCGAGTGAATAAACAATATCGTTTA
ATTTTTAAATATGAAAATAACGAAGTAAATAATTTATATTTAGATCCACATTCTTATAATTTGTAA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 12347.17 Da
• Isoelectric Point: 6.8804

>AT36602 WP_005650217.1 NZ_AP022867:1331359-1331682 [Haemophilus influenzae]
MMTRKPTSVGEILQEEFLEPLSLKISDLAQILDVHRNTASNIVNNSSRITLEMAVKLAKVFDTTPEFWLNLQTRIDLWDL
EHNKRFQQSLANVKPALHRHDTSTFAM

Nucleotide

• Length: 324 bp

>AT36602 NZ_AP022867:1331359-1331682 [Haemophilus influenzae]
ATGATGACGAGAAAACCCACCTCTGTTGGTGAAATTCTACAAGAAGAGTTTTTAGAACCTTTAAGTCTTAAAATTAGTGA
TTTAGCTCAAATTTTAGATGTACATCGTAATACGGCAAGCAATATTGTTAATAATAGTTCACGAATTACGCTAGAAATGG
CAGTTAAACTTGCTAAAGTATTTGATACTACGCCTGAGTTTTGGCTTAATCTGCAAACACGTATTGATTTATGGGACCTT
GAACACAATAAACGCTTTCAACAAAGTCTTGCAAATGTTAAGCCCGCTCTACATCGGCATGATACTTCTACATTTGCAAT
GTAA

Structures

Toxin

• AlphaFold DB: A0A806EC70