Detailed information of TA system TA036555

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/Txe-RelB
• Location: 58854..59359
• Replicon: plasmid pVE80-1
• Accession: NZ_AP022823
• Organism: Enterococcus saigonensis strain VE80

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: -
• Locus tag: EsVE80_RS13520
• Protein ID: WP_161841584.1
• Coordinates: 59093..59359 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: U5SCD4
• Locus tag: EsVE80_RS13515
• Protein ID: WP_010620828.1
• Coordinates: 58854..59108 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EsVE80_RS13490	54092..56017	-	1926	WP_173104235.1	heavy metal translocating P-type ATPase	-
EsVE80_RS13495	56316..56630	+	315	WP_068561834.1	metalloregulator ArsR/SmtB family transcription factor	-
EsVE80_RS13500	56674..57582	+	909	WP_068561832.1	cation diffusion facilitator family transporter	-
EsVE80_RS13505	57682..58293	-	612	WP_034536057.1	CadD family cadmium resistance transporter	-
EsVE80_RS13510	58310..58672	-	363	WP_068561828.1	metalloregulator ArsR/SmtB family transcription factor	-
EsVE80_RS13515	58854..59108	+	255	WP_010620828.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
EsVE80_RS13520	59093..59359	+	267	WP_161841584.1	Txe/YoeB family addiction module toxin	Toxin
EsVE80_RS13525	59570..60187	-	618	WP_173104236.1	CadD family cadmium resistance transporter	-
EsVE80_RS13530	60204..60566	-	363	WP_173104237.1	metalloregulator ArsR/SmtB family transcription factor	-
EsVE80_RS13535	60738..61418	-	681	WP_173104238.1	IS6-like element IS1216 family transposase	-
EsVE80_RS13540	61544..61849	-	306	WP_010711855.1	hypothetical protein	-
EsVE80_RS13545	61883..62380	-	498	WP_002300565.1	molecular chaperone DnaJ	-
EsVE80_RS13550	62912..63229	-	318	WP_002326830.1	hypothetical protein	-
EsVE80_RS13555	63669..63785	-	117	WP_173104241.1	zeta-toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	VanHAX / erm(B)	-	1..76008	76008
	-	flank	IS/Tn	-	-	60738..61424	686

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (13-86)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-62)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10398.98 Da
• Isoelectric Point: 10.1706

>T36555 WP_161841584.1 NZ_AP022823:59093-59359 [Enterococcus saigonensis]
MGYSVKLAKSATKDLKHLKGAHLDQKFKNLMEVLKENPFQNPPPYEKLVGNLKDKYSRRLNIQHRVVYSVDNDAKEVIIW
STWTHYER

Nucleotide

• Length: 267 bp

>T36555 NZ_AP022823:59093-59359 [Enterococcus saigonensis]
TTGGGATACTCTGTAAAATTAGCAAAATCAGCTACAAAAGACCTGAAACATTTAAAAGGGGCTCATTTAGATCAAAAATT
TAAAAATCTCATGGAAGTTTTAAAGGAGAATCCGTTCCAAAATCCCCCGCCTTATGAGAAATTAGTCGGAAATTTAAAAG
ATAAATATTCACGTCGTTTAAATATTCAGCACCGGGTGGTTTATTCGGTGGATAATGACGCCAAAGAAGTCATTATTTGG
TCTACTTGGACGCACTATGAAAGATAG

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9673.80 Da
• Isoelectric Point: 4.1473

>AT36555 WP_010620828.1 NZ_AP022823:58854-59108 [Enterococcus saigonensis]
MSIVNPSQARKVFYQLLKDVNKTNEPIYISGKNEESEAVMVSKKDWDAIQETLYLQSAGVADVIHQREQENEFVALEDID
WDTL

Nucleotide

• Length: 255 bp

>AT36555 NZ_AP022823:58854-59108 [Enterococcus saigonensis]
ATGTCTATCGTAAACCCTAGCCAAGCTAGAAAAGTCTTTTATCAACTATTGAAAGACGTCAACAAAACTAACGAACCCAT
TTATATCTCTGGCAAAAACGAAGAAAGTGAAGCAGTTATGGTCAGTAAAAAAGACTGGGACGCAATTCAAGAAACATTAT
ACCTTCAATCTGCTGGCGTAGCAGATGTGATCCACCAGCGTGAACAAGAAAATGAGTTTGTTGCATTGGAGGATATCGAT
TGGGATACTCTGTAA

Structures

Antitoxin

• AlphaFold DB: A0A1H6W4Y2