Detailed information of TA system TA036554

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3579591..3580222
• Replicon: chromosome
• Accession: NZ_AP022821
• Organism: Halomonas meridiana strain Slthf1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A3A1Z2D9
• Locus tag: HMSLTHF_RS16835
• Protein ID: WP_044628576.1
• Coordinates: 3579591..3579884 (+)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HMSLTHF_RS16840
• Protein ID: WP_172416710.1
• Coordinates: 3579899..3580222 (+)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HMSLTHF_RS16810	3574771..3575466	-	696	WP_044628581.1	helix-turn-helix transcriptional regulator	-
HMSLTHF_RS16815	3575752..3576360	+	609	WP_054641669.1	RdgB/HAM1 family non-canonical purine NTP pyrophosphatase	-
HMSLTHF_RS16820	3576353..3577522	+	1170	WP_054641670.1	radical SAM family heme chaperone HemW	-
HMSLTHF_RS16825	3577601..3578272	+	672	WP_062361041.1	OmpA family protein	-
HMSLTHF_RS16830	3578358..3579203	+	846	WP_054641671.1	23S rRNA (adenine(2030)-N(6))-methyltransferase RlmJ	-
HMSLTHF_RS16835	3579591..3579884	+	294	WP_044628576.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HMSLTHF_RS16840	3579899..3580222	+	324	WP_172416710.1	HigA family addiction module antidote protein	Antitoxin
HMSLTHF_RS16845	3580675..3581781	-	1107	WP_044628573.1	FUSC family protein	-
HMSLTHF_RS16850	3581912..3582436	-	525	WP_197931406.1	cytochrome b/b6 domain-containing protein	-
HMSLTHF_RS16855	3582530..3583480	-	951	WP_007105033.1	aldo/keto reductase	-
HMSLTHF_RS16860	3583544..3584338	-	795	WP_013786623.1	SDR family oxidoreductase	-
HMSLTHF_RS16865	3584384..3585205	-	822	WP_172416711.1	dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3570831..3582511	11680

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (2-94)

Antitoxin

• Cro/C1-type helix-turn-helix domain (19-69)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 11385.80 Da
• Isoelectric Point: 5.6202

>T36554 WP_044628576.1 NZ_AP022821:3579591-3579884 [Halomonas meridiana]
MAVEFRDIWLEAFYDEDQSHKKIPSTIEGALFRKIQILDAATQESDLRIPPGNRFEHLQGNLSGWCSIRVNKQYRLIFRW
EDGTAQDTYLDPHVYKG

Nucleotide

• Length: 294 bp

>T36554 NZ_AP022821:3579591-3579884 [Halomonas meridiana]
ATGGCGGTTGAATTCAGGGATATATGGCTGGAGGCTTTCTACGACGAAGACCAAAGCCACAAGAAAATACCCAGCACTAT
TGAAGGCGCTCTGTTCAGAAAGATTCAGATTCTGGATGCCGCGACGCAGGAGTCAGACTTGAGGATTCCGCCGGGTAACC
GTTTTGAACATTTGCAGGGAAACCTTTCTGGATGGTGTTCCATTCGGGTTAACAAACAGTATCGCCTGATTTTTCGCTGG
GAAGATGGCACCGCCCAAGATACATATCTAGACCCGCATGTTTACAAGGGCTAG

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 12166.23 Da
• Isoelectric Point: 10.3987

>AT36554 WP_172416710.1 NZ_AP022821:3579899-3580222 [Halomonas meridiana]
MRNTKRRPVTVGEMLVSEFLEPMKIEISELAEAMGVHRNTLSRIAHDKGTLTAPMAIKLAAALGNTPEFWLNIQHAVELW
DVRHRAYEQEAKNVKRVKPQVLPLKQA

Nucleotide

• Length: 324 bp

>AT36554 NZ_AP022821:3579899-3580222 [Halomonas meridiana]
ATGAGGAATACAAAACGTCGTCCAGTGACAGTTGGCGAAATGCTGGTCAGCGAGTTTCTGGAGCCGATGAAAATAGAAAT
CAGTGAACTGGCCGAAGCAATGGGTGTGCACAGAAATACACTCAGCCGGATTGCACATGACAAAGGCACGCTGACAGCAC
CCATGGCCATCAAACTCGCAGCGGCTTTGGGGAATACGCCGGAGTTCTGGCTGAATATTCAGCACGCGGTTGAGTTATGG
GATGTTCGTCATCGTGCCTATGAGCAGGAAGCGAAAAATGTGAAACGGGTAAAGCCGCAAGTACTGCCACTAAAGCAGGC
CTGA

Structures

Toxin

• AlphaFold DB: A0A3A1Z2D9