Detailed information of TA system TA036545

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 43609..44255
• Replicon: plasmid JE86-ST05_p3
• Accession: NZ_AP022818
• Organism: Escherichia coli strain JE86-ST05

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7R6ZH56
• Locus tag: JL003_RS27715
• Protein ID: WP_000269913.1
• Coordinates: 43908..44255 (-)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JL003_RS27710
• Protein ID: WP_137519595.1
• Coordinates: 43609..43908 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JL003_RS27670	38803..39051	+	249	WP_000730008.1	hypothetical protein	-
JL003_RS27675	39866..40121	+	256	Protein_57	ash family protein	-
JL003_RS27680	40168..40359	+	192	WP_000183351.1	hypothetical protein	-
JL003_RS27685	40356..41498	+	1143	WP_080855624.1	ORF6N domain-containing protein	-
JL003_RS27690	41509..41697	-	189	WP_004105254.1	hypothetical protein	-
JL003_RS27695	42308..42580	-	273	WP_000148349.1	helix-turn-helix domain-containing protein	-
JL003_RS27700	42644..43024	-	381	WP_137519594.1	hypothetical protein	-
JL003_RS27705	43291..43575	+	285	WP_001673380.1	hypothetical protein	-
JL003_RS27710	43609..43908	-	300	WP_137519595.1	XRE family transcriptional regulator	Antitoxin
JL003_RS27715	43908..44255	-	348	WP_000269913.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JL003_RS27720	44500..44763	-	264	WP_000424604.1	hypothetical protein	-
JL003_RS27725	44787..45074	-	288	WP_000356589.1	hypothetical protein	-
JL003_RS27730	45718..46554	-	837	WP_024189035.1	replication initiator protein RepA	-
JL003_RS27735	46880..47434	-	555	WP_001199265.1	recombinase family protein	-
JL003_RS27740	47570..48412	+	843	WP_137519596.1	phage tail protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..48666	48666

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (32-80)

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 13486.53 Da
• Isoelectric Point: 9.8723

>T36545 WP_000269913.1 NZ_AP022818:c44255-43908 [Escherichia coli]
MWTVLFSQRFDGWLNEQEDALQEKVLADLKKLQVYGPELPRPYADTVKGSRYKNMKELRVQFSGRPIRAFYAFDPIRRAI
VLCAGDKSNDKRFYKKLVRIAEDEFAAHLNTLESK

Nucleotide

• Length: 348 bp

>T36545 NZ_AP022818:c44255-43908 [Escherichia coli]
ATGTGGACGGTACTTTTTAGCCAGCGATTTGACGGCTGGCTAAATGAGCAAGAAGACGCTCTTCAGGAAAAGGTTTTGGC
TGATTTGAAAAAGCTACAGGTTTACGGACCTGAGTTACCCAGGCCATATGCCGATACCGTAAAAGGTTCTCGGTACAAAA
ATATGAAAGAGCTTCGCGTTCAGTTTTCTGGCCGTCCGATAAGAGCCTTTTATGCGTTCGATCCGATTCGCCGGGCTATC
GTTCTTTGTGCAGGAGACAAAAGTAACGATAAGCGGTTTTATAAAAAACTGGTGCGTATAGCTGAGGATGAGTTTGCAGC
ACATCTGAACACACTGGAGAGCAAGTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11097.96 Da
• Isoelectric Point: 5.8899

>AT36545 WP_137519595.1 NZ_AP022818:c43908-43609 [Escherichia coli]
MRTLDEVIASRSPESQARIKEMADEMILEVGLQMMREELQLSQKQVAEVMGISQPAVTKLEQRGNDLKLATLKRYVEAMG
GKLSLDVELPTGKRVAFHV

Nucleotide

• Length: 300 bp

>AT36545 NZ_AP022818:c43908-43609 [Escherichia coli]
ATGAGAACATTAGATGAGGTGATTGCCAGCCGTTCACCGGAAAGCCAGGCGCGAATTAAAGAAATGGCAGATGAGATGAT
TCTTGAGGTCGGCTTGCAGATGATGCGTGAAGAACTCCAGTTATCACAAAAGCAGGTTGCTGAGGTGATGGGTATAAGCC
AGCCAGCAGTAACAAAGCTGGAGCAGCGCGGAAATGATTTAAAGCTGGCGACATTAAAGCGTTACGTTGAAGCTATGGGA
GGCAAATTAAGCCTGGATGTTGAGCTTCCAACAGGAAAGCGAGTTGCATTCCATGTCTAA

Structures

Toxin

• AlphaFold DB: A0A7R6ZH56