Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2553380..2553882 Replicon chromosome
Accession NZ_AP022815
Organism Escherichia coli strain JE86-ST05

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag JL003_RS12965 Protein ID WP_000767829.1
Coordinates 2553380..2553634 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag JL003_RS12970 Protein ID WP_001259255.1
Coordinates 2553631..2553882 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JL003_RS12935 2548395..2548622 - 228 WP_000234896.1 sulfurtransferase TusA -
JL003_RS12940 2548636..2549694 - 1059 WP_000492339.1 transport protein YeeE -
JL003_RS12945 2549873..2551231 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
JL003_RS12950 2551221..2551283 - 63 WP_010723108.1 membrane protein YoeI -
JL003_RS12955 2551498..2552427 - 930 WP_000803351.1 LysR family transcriptional regulator -
JL003_RS12960 2552473..2553297 - 825 WP_000754737.1 SDR family oxidoreductase -
JL003_RS12965 2553380..2553634 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
JL003_RS12970 2553631..2553882 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
JL003_RS12975 2554165..2554215 + 51 WP_001364200.1 his operon leader peptide -
JL003_RS12980 2554361..2555260 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
JL003_RS12985 2555266..2556570 + 1305 WP_001548748.1 histidinol dehydrogenase -
JL003_RS12990 2556567..2557637 + 1071 WP_032329361.1 histidinol-phosphate transaminase -
JL003_RS12995 2557637..2558704 + 1068 WP_000080093.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T36531 WP_000767829.1 NZ_AP022815:c2553634-2553380 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T36531 NZ_AP022815:c2553634-2553380 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT36531 WP_001259255.1 NZ_AP022815:c2553882-2553631 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT36531 NZ_AP022815:c2553882-2553631 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References