Detailed information of TA system TA036516

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 822..1468
• Replicon: plasmid JE86-ST02_p3
• Accession: NZ_AP022814
• Organism: Escherichia coli strain JE86-ST02

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7R6ZH56
• Locus tag: JLZ98_RS27230
• Protein ID: WP_000269913.1
• Coordinates: 822..1169 (+)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JLZ98_RS27235
• Protein ID: WP_137519595.1
• Coordinates: 1169..1468 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JLZ98_RS27220	3..290	+	288	WP_000356589.1	hypothetical protein	-
JLZ98_RS27225	314..577	+	264	WP_000424604.1	hypothetical protein	-
JLZ98_RS27230	822..1169	+	348	WP_000269913.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JLZ98_RS27235	1169..1468	+	300	WP_137519595.1	XRE family transcriptional regulator	Antitoxin
JLZ98_RS27240	1502..1786	-	285	WP_001673380.1	hypothetical protein	-
JLZ98_RS27245	2053..2433	+	381	WP_137519594.1	hypothetical protein	-
JLZ98_RS27250	2497..2769	+	273	WP_000148349.1	helix-turn-helix domain-containing protein	-
JLZ98_RS27255	3380..3568	+	189	WP_004105254.1	hypothetical protein	-
JLZ98_RS27260	3579..4721	-	1143	WP_080855624.1	ORF6N domain-containing protein	-
JLZ98_RS27265	4718..4909	-	192	WP_000183351.1	hypothetical protein	-
JLZ98_RS27270	4956..5211	-	256	Protein_10	ash family protein	-
JLZ98_RS27275	6026..6274	-	249	WP_000730008.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..48397	48397

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (32-80)

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 13486.53 Da
• Isoelectric Point: 9.8723

>T36516 WP_000269913.1 NZ_AP022814:822-1169 [Escherichia coli]
MWTVLFSQRFDGWLNEQEDALQEKVLADLKKLQVYGPELPRPYADTVKGSRYKNMKELRVQFSGRPIRAFYAFDPIRRAI
VLCAGDKSNDKRFYKKLVRIAEDEFAAHLNTLESK

Nucleotide

• Length: 348 bp

>T36516 NZ_AP022814:822-1169 [Escherichia coli]
ATGTGGACGGTACTTTTTAGCCAGCGATTTGACGGCTGGCTAAATGAGCAAGAAGACGCTCTTCAGGAAAAGGTTTTGGC
TGATTTGAAAAAGCTACAGGTTTACGGACCTGAGTTACCCAGGCCATATGCCGATACCGTAAAAGGTTCTCGGTACAAAA
ATATGAAAGAGCTTCGCGTTCAGTTTTCTGGCCGTCCGATAAGAGCCTTTTATGCGTTCGATCCGATTCGCCGGGCTATC
GTTCTTTGTGCAGGAGACAAAAGTAACGATAAGCGGTTTTATAAAAAACTGGTGCGTATAGCTGAGGATGAGTTTGCAGC
ACATCTGAACACACTGGAGAGCAAGTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11097.96 Da
• Isoelectric Point: 5.8899

>AT36516 WP_137519595.1 NZ_AP022814:1169-1468 [Escherichia coli]
MRTLDEVIASRSPESQARIKEMADEMILEVGLQMMREELQLSQKQVAEVMGISQPAVTKLEQRGNDLKLATLKRYVEAMG
GKLSLDVELPTGKRVAFHV

Nucleotide

• Length: 300 bp

>AT36516 NZ_AP022814:1169-1468 [Escherichia coli]
ATGAGAACATTAGATGAGGTGATTGCCAGCCGTTCACCGGAAAGCCAGGCGCGAATTAAAGAAATGGCAGATGAGATGAT
TCTTGAGGTCGGCTTGCAGATGATGCGTGAAGAACTCCAGTTATCACAAAAGCAGGTTGCTGAGGTGATGGGTATAAGCC
AGCCAGCAGTAACAAAGCTGGAGCAGCGCGGAAATGATTTAAAGCTGGCGACATTAAAGCGTTACGTTGAAGCTATGGGA
GGCAAATTAAGCCTGGATGTTGAGCTTCCAACAGGAAAGCGAGTTGCATTCCATGTCTAA

Structures

Toxin

• AlphaFold DB: A0A7R6ZH56