Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2528404..2528906 Replicon chromosome
Accession NZ_AP022811
Organism Escherichia coli strain JE86-ST02

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag JLZ98_RS12830 Protein ID WP_000767829.1
Coordinates 2528404..2528658 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag JLZ98_RS12835 Protein ID WP_001259255.1
Coordinates 2528655..2528906 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JLZ98_RS12800 2523419..2523646 - 228 WP_000234896.1 sulfurtransferase TusA -
JLZ98_RS12805 2523660..2524718 - 1059 WP_000492339.1 transport protein YeeE -
JLZ98_RS12810 2524897..2526255 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
JLZ98_RS12815 2526245..2526307 - 63 WP_010723108.1 membrane protein YoeI -
JLZ98_RS12820 2526522..2527451 - 930 WP_000803351.1 LysR family transcriptional regulator -
JLZ98_RS12825 2527497..2528321 - 825 WP_000754737.1 SDR family oxidoreductase -
JLZ98_RS12830 2528404..2528658 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
JLZ98_RS12835 2528655..2528906 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
JLZ98_RS12840 2529189..2529239 + 51 WP_001364200.1 his operon leader peptide -
JLZ98_RS12845 2529385..2530284 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
JLZ98_RS12850 2530290..2531594 + 1305 WP_001548748.1 histidinol dehydrogenase -
JLZ98_RS12855 2531591..2532661 + 1071 WP_032329361.1 histidinol-phosphate transaminase -
JLZ98_RS12860 2532661..2533728 + 1068 WP_000080093.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T36502 WP_000767829.1 NZ_AP022811:c2528658-2528404 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T36502 NZ_AP022811:c2528658-2528404 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT36502 WP_001259255.1 NZ_AP022811:c2528906-2528655 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT36502 NZ_AP022811:c2528906-2528655 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References