Detailed information of TA system TA036474

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): symE-agrB/SymE(toxin)
• Location: 5011631..5012043
• Replicon: chromosome
• Accession: NZ_AP022650
• Organism: Escherichia coli strain 09-02E

Toxin (Protein)

• Gene name: symE
• Uniprot ID: S1NWQ7
• Locus tag: HRS14_RS24380
• Protein ID: WP_000132614.1
• Coordinates: 5011631..5011972 (-)
• Length: 114 a.a.

Antitoxin (RNA)

Gene name	agrB
Locus tag	-
Coordinates	5011967..5012043 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HRS14_RS24360	5006700..5007980	-	1281	WP_001338077.1	DUF445 domain-containing protein	-
HRS14_RS24365	5008165..5009085	+	921	WP_001513532.1	Rpn family recombination-promoting nuclease/putative transposase	-
HRS14_RS24370	5009313..5009393	+	81	WP_020233658.1	hypothetical protein	-
HRS14_RS24375	5009428..5011584	+	2157	WP_001513533.1	DUF262 domain-containing protein	-
HRS14_RS24380	5011631..5011972	-	342	WP_000132614.1	endoribonuclease SymE	Toxin
-	5011967..5012043	+	77	NuclAT_8	-	Antitoxin
-	5011967..5012043	+	77	NuclAT_8	-	Antitoxin
-	5011967..5012043	+	77	NuclAT_8	-	Antitoxin
-	5011967..5012043	+	77	NuclAT_8	-	Antitoxin
-	5011967..5012043	+	77	NuclAT_9	-	Antitoxin
-	5011967..5012043	+	77	NuclAT_9	-	Antitoxin
-	5011967..5012043	+	77	NuclAT_9	-	Antitoxin
-	5011967..5012043	+	77	NuclAT_9	-	Antitoxin
HRS14_RS24385	5012193..5013962	-	1770	WP_001513535.1	restriction endonuclease subunit S	-
HRS14_RS24390	5013962..5015431	-	1470	WP_001387312.1	type I restriction-modification system subunit M	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SymE-like (20-71)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12266.07 Da
• Isoelectric Point: 8.4982

>T36474 WP_000132614.1 NZ_AP022650:c5011972-5011631 [Escherichia coli]
MTDTHSIAQPFEAEVSPANNRQLTVSYASRYPDYSRIPAITLKGQWLEAAGFATGTAVDVKVMEGCIVLTAQPPAAAESE
LMQSLRQVCKLSARKQRQVQEFIGVIAGKQKVA

Nucleotide

• Length: 342 bp

>T36474 NZ_AP022650:c5011972-5011631 [Escherichia coli]
ATGACTGACACGCATTCTATTGCACAACCGTTCGAAGCAGAAGTCTCCCCGGCAAATAACCGTCAATTAACCGTGAGTTA
TGCGAGTCGCTACCCGGATTACAGCCGTATTCCCGCCATCACCCTGAAAGGTCAGTGGCTGGAAGCCGCCGGTTTTGCCA
CCGGCACTGCGGTAGATGTCAAAGTAATGGAAGGCTGTATTGTCCTCACCGCCCAACCACCCGCCGCCGCCGAGAGCGAA
CTGATGCAGTCGCTGCGCCAGGTGTGCAAGCTGTCGGCGCGTAAACAAAGGCAGGTGCAGGAGTTTATTGGGGTGATTGC
GGGTAAACAGAAAGTCGCCTGA

Antitoxin

Nucleotide

• Length: 77 bp

>AT36474 NZ_AP022650:5011967-5012043 [Escherichia coli]
AGTCATAACTGCTATTCCCTATAAATAGTGATTGTGATTAGCGGTGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Toxin

• AlphaFold DB: S1NWQ7

Antitoxin