Detailed information of TA system TA036470

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4406206..4406808
• Replicon: chromosome
• Accession: NZ_AP022650
• Organism: Escherichia coli strain 09-02E

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: HRS14_RS21510
• Protein ID: WP_000897302.1
• Coordinates: 4406206..4406517 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HRS14_RS21515
• Protein ID: WP_000356397.1
• Coordinates: 4406518..4406808 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HRS14_RS21480	4401236..4402021	+	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-
HRS14_RS21485	4402120..4402719	+	600	WP_001314324.1	glucose-1-phosphatase	-
HRS14_RS21490	4402713..4403585	+	873	WP_000920762.1	virulence factor BrkB family protein	-
HRS14_RS21495	4403582..4404019	+	438	WP_000560978.1	D-tyrosyl-tRNA(Tyr) deacylase	-
HRS14_RS21500	4404064..4405005	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
HRS14_RS21505	4405069..4405977	-	909	WP_001774092.1	alpha/beta hydrolase	-
HRS14_RS21510	4406206..4406517	+	312	WP_000897302.1	hypothetical protein	Toxin
HRS14_RS21515	4406518..4406808	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
HRS14_RS21520	4407167..4407445	+	279	WP_001296612.1	hypothetical protein	-
HRS14_RS21525	4407841..4408059	+	219	WP_001314326.1	ribbon-helix-helix domain-containing protein	-
HRS14_RS21530	4408275..4409204	-	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
HRS14_RS21535	4409201..4409836	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
HRS14_RS21540	4409833..4410735	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T36470 WP_000897302.1 NZ_AP022650:4406206-4406517 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T36470 NZ_AP022650:4406206-4406517 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT36470 WP_000356397.1 NZ_AP022650:4406518-4406808 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT36470 NZ_AP022650:4406518-4406808 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGTACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: S1P416