Detailed information of TA system TA036467

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mqsRA (relBE)/MqsR-MqsA
• Location: 3456552..3457245
• Replicon: chromosome
• Accession: NZ_AP022650
• Organism: Escherichia coli strain 09-02E

Toxin (Protein)

• Gene name: mqsR
• Uniprot ID: S1EZG2
• Locus tag: HRS14_RS16980
• Protein ID: WP_000415584.1
• Coordinates: 3456949..3457245 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: mqsA
• Uniprot ID: S1EBV2
• Locus tag: HRS14_RS16975
• Protein ID: WP_000650107.1
• Coordinates: 3456552..3456947 (-)
• Length: 132 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HRS14_RS16965	3452416..3454674	-	2259	WP_001281841.1	DNA topoisomerase IV subunit A	-
HRS14_RS16970	3454812..3456419	-	1608	WP_001295629.1	ABC transporter substrate-binding protein	-
HRS14_RS16975	3456552..3456947	-	396	WP_000650107.1	type II toxin-antitoxin system antitoxin MqsA	Antitoxin
HRS14_RS16980	3456949..3457245	-	297	WP_000415584.1	type II toxin-antitoxin system toxin MqsR	Toxin
HRS14_RS16985	3457450..3457932	-	483	WP_000183505.1	transcriptional regulator	-
HRS14_RS16990	3457985..3458377	-	393	WP_000712658.1	OB fold stress tolerance protein YgiW	-
HRS14_RS16995	3458529..3459188	+	660	WP_001221493.1	two-component system response regulator QseB	-
HRS14_RS17000	3459185..3460534	+	1350	WP_000673402.1	two-component system sensor histidine kinase QseC	-
HRS14_RS17005	3460580..3460912	-	333	WP_000917685.1	DUF2645 family protein	-
HRS14_RS17010	3461231..3461812	+	582	WP_000065430.1	NADPH:quinone oxidoreductase MdaB	-
HRS14_RS17015	3461843..3462157	+	315	WP_000958598.1	antibiotic biosynthesis monooxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3448089..3457245	9156

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin MqsR (5-97)

Antitoxin

• Zinc finger, MqsA-type (3-47)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11231.96 Da
• Isoelectric Point: 8.9070

>T36467 WP_000415584.1 NZ_AP022650:c3457245-3456949 [Escherichia coli]
MEKRTPHTRLSQVKKLVNAGQVRTTRSALLNADELGLDFDGMCNVIIGLSESDFYKSMTTYSDHTIWQDVYRPRLVTGQV
YLKITVIHDVLIVSFKEK

Nucleotide

• Length: 297 bp

>T36467 NZ_AP022650:c3457245-3456949 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCCCTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin

Protein

• Length: 132 a.a.
• Molecular weight: 14703.10 Da
• Isoelectric Point: 9.2136

>AT36467 WP_000650107.1 NZ_AP022650:c3456947-3456552 [Escherichia coli]
MKCPVCHQGEMVSGIKDIPYTFRGRKTVLKGIHGLYCVHCEESIMNKEESDAFMAQVKAFRASVNAETVAPEFIVKVRKK
LSLTQKEASEIFGGGVNAFSRYEKGNAQPHPSTIKLLRVLDKHPELLNEIR

Nucleotide

• Length: 396 bp

>AT36467 NZ_AP022650:c3456947-3456552 [Escherichia coli]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Structures

Toxin

• PDB: 3HI2

Antitoxin

• AlphaFold DB: A0A7U9LU65