Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2284935..2285437 Replicon chromosome
Accession NZ_AP022650
Organism Escherichia coli strain 09-02E

Toxin (Protein)


Gene name yoeB Uniprot ID S1P977
Locus tag HRS14_RS11565 Protein ID WP_000767822.1
Coordinates 2284935..2285189 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag HRS14_RS11570 Protein ID WP_001259255.1
Coordinates 2285186..2285437 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HRS14_RS11545 2281428..2282786 - 1359 WP_000019194.1 putrescine/proton symporter PlaP -
HRS14_RS11550 2282776..2282838 - 63 WP_010723108.1 membrane protein YoeI -
HRS14_RS11555 2283053..2283982 - 930 WP_000803366.1 LysR family transcriptional regulator -
HRS14_RS11560 2284028..2284852 - 825 WP_000754737.1 SDR family oxidoreductase -
HRS14_RS11565 2284935..2285189 - 255 WP_000767822.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
HRS14_RS11570 2285186..2285437 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
HRS14_RS11575 2285720..2285770 + 51 WP_001364200.1 his operon leader peptide -
HRS14_RS11580 2285916..2286815 + 900 WP_000131779.1 ATP phosphoribosyltransferase -
HRS14_RS11585 2286821..2288125 + 1305 WP_001445483.1 histidinol dehydrogenase -
HRS14_RS11590 2288122..2289192 + 1071 WP_001538918.1 histidinol-phosphate transaminase -
HRS14_RS11595 2289192..2290259 + 1068 WP_001538920.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T36464 WP_000767822.1 NZ_AP022650:c2285189-2284935 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINEIIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T36464 NZ_AP022650:c2285189-2284935 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAAATTATCAAAGATACACGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT36464 WP_001259255.1 NZ_AP022650:c2285437-2285186 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT36464 NZ_AP022650:c2285437-2285186 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1P977

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References