Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1667904..1668466 Replicon chromosome
Accession NZ_AP022650
Organism Escherichia coli strain 09-02E

Toxin (Protein)


Gene name higB Uniprot ID S1Q1V8
Locus tag HRS14_RS08315 Protein ID WP_000605675.1
Coordinates 1668188..1668466 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag HRS14_RS08310 Protein ID WP_000781370.1
Coordinates 1667904..1668188 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HRS14_RS08295 1663085..1666132 + 3048 WP_011478186.1 formate dehydrogenase-N subunit alpha -
HRS14_RS08300 1666145..1667029 + 885 WP_001240584.1 formate dehydrogenase N subunit beta -
HRS14_RS08305 1667022..1667675 + 654 WP_000045647.1 formate dehydrogenase-N subunit gamma -
HRS14_RS08310 1667904..1668188 - 285 WP_000781370.1 HigA family addiction module antidote protein Antitoxin
HRS14_RS08315 1668188..1668466 - 279 WP_000605675.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
HRS14_RS08320 1668652..1669662 - 1011 WP_000642412.1 alcohol dehydrogenase AdhP -
HRS14_RS08325 1669796..1671493 - 1698 WP_000433460.1 malate dehydrogenase -
HRS14_RS08330 1671650..1671787 - 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
HRS14_RS08335 1671889..1672104 - 216 WP_000495766.1 biofilm-dependent modulation protein -
HRS14_RS08340 1672449..1672880 + 432 WP_000152305.1 peroxiredoxin OsmC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10537.16 Da        Isoelectric Point: 7.3206

>T36455 WP_000605675.1 NZ_AP022650:c1668466-1668188 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T36455 NZ_AP022650:c1668466-1668188 [Escherichia coli]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTGTTTCTTCTCGGCAAAACCTCCGGCGTTATACCGACGCAAGT
CAAACGATTACGCCACCGACTCGCTGTGATTGATGCAGCATGCTGTCTTGCTGATATCGATATGCCCGGTTACCGACTAC
ATCCGTTAAGCGGCGATCGCGATGGAATTTGGGCGATATCTGTCTCGGGCAACTGGCGAATCACATTTGAATTCGTCAAT
GGCGATGCATACATACTGGATTACGAGGACTATCACTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT36455 WP_000781370.1 NZ_AP022650:c1668188-1667904 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT36455 NZ_AP022650:c1668188-1667904 [Escherichia coli]
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAACTTAATGTCAGCCTGCGCGAGTT
TGCCAGAGCAATGGAAATTGCGCCCTCAACGGCAAGCCGATTGCTGACCGGAAAAGCAGCTTTGACGCCAGAAATGGCAA
TAAAACTTTCCGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTGCAAAATGCCTGGAGTCTGGCAGAGGCAGAA
AAAACAGTGGATGTGTCGCGACTGCGCCGTCTGGTTACGCAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1Q1V8

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References