Detailed information of TA system TA036451

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 326916..327610
• Replicon: chromosome
• Accession: NZ_AP022650
• Organism: Escherichia coli strain 09-02E

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1PJQ2
• Locus tag: HRS14_RS01535
• Protein ID: WP_001263500.1
• Coordinates: 327212..327610 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: HRS14_RS01530
• Protein ID: WP_000554758.1
• Coordinates: 326916..327209 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HRS14_RS01505	322743..323210	+	468	WP_000725261.1	flagellar basal body-associated FliL family protein	-
HRS14_RS01510	323230..323946	+	717	WP_000938731.1	FliA/WhiG family RNA polymerase sigma factor	-
HRS14_RS01515	323959..324822	+	864	WP_001169518.1	flagellar motor stator protein MotA	-
HRS14_RS01520	324825..325748	+	924	WP_001532973.1	putative lateral flagellar export/assembly protein LafU	-
HRS14_RS01525	325819..326864	+	1046	Protein_299	DNA polymerase IV	-
HRS14_RS01530	326916..327209	+	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
HRS14_RS01535	327212..327610	+	399	WP_001263500.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
HRS14_RS01540	327620..328072	+	453	WP_001059895.1	GNAT family N-acetyltransferase	-
HRS14_RS01545	328262..329401	+	1140	WP_000521561.1	RNA ligase RtcB family protein	-
HRS14_RS01550	329398..330012	+	615	WP_000602124.1	peptide chain release factor H	-
HRS14_RS01555	330069..331526	-	1458	WP_001292999.1	cytosol nonspecific dipeptidase	-
HRS14_RS01560	331787..332245	+	459	WP_001291991.1	xanthine phosphoribosyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15413.85 Da
• Isoelectric Point: 8.9511

>T36451 WP_001263500.1 NZ_AP022650:327212-327610 [Escherichia coli]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDAAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T36451 NZ_AP022650:327212-327610 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGAAGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGTGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGCAGCGCATTTGGTATATTGTCAGGGCGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT36451 WP_000554758.1 NZ_AP022650:326916-327209 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT36451 NZ_AP022650:326916-327209 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
TGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTTGAAGCATTAATGGACATGCTTG
CAGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCGAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: S1PJQ2

Antitoxin

• AlphaFold DB: S1QAE3