Detailed information of TA system TA036440

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3831778..3832379
• Replicon: chromosome
• Accession: NZ_AP022639
• Organism: Bradyrhizobium diazoefficiens strain H12S4

Toxin (Protein)

• Gene name: graT
• Uniprot ID: Q89QW2
• Locus tag: H7K75_RS17230
• Protein ID: WP_011085796.1
• Coordinates: 3832098..3832379 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: H7K75_RS17225
• Protein ID: WP_011085795.1
• Coordinates: 3831778..3832086 (-)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7K75_RS17210	3827024..3829612	+	2589	WP_011085792.1	MMPL family transporter	-
H7K75_RS17215	3829580..3830236	-	657	WP_038965759.1	DUF2147 domain-containing protein	-
H7K75_RS17220	3830378..3831769	+	1392	WP_011085794.1	aminobacteriohopanetriol synthase HpnO	-
H7K75_RS17225	3831778..3832086	-	309	WP_011085795.1	HigA family addiction module antidote protein	Antitoxin
H7K75_RS17230	3832098..3832379	-	282	WP_011085796.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
H7K75_RS17235	3832451..3833218	-	768	WP_011085797.1	anti-sigma factor	-
H7K75_RS17240	3833215..3833763	-	549	WP_028172321.1	sigma-70 family RNA polymerase sigma factor	-
H7K75_RS17245	3833891..3834226	-	336	WP_011085799.1	cupredoxin family copper-binding protein	-
H7K75_RS17250	3834252..3835196	-	945	WP_182872536.1	metallophosphoesterase	-
H7K75_RS17255	3835376..3836242	+	867	WP_011085801.1	helix-turn-helix transcriptional regulator	-
H7K75_RS17260	3836265..3836588	-	324	WP_011085802.1	antibiotic biosynthesis monooxygenase	-
H7K75_RS17265	3836634..3836996	-	363	WP_011085803.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (19-67)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10528.99 Da
• Isoelectric Point: 9.7579

>T36440 WP_011085796.1 NZ_AP022639:c3832379-3832098 [Bradyrhizobium diazoefficiens]
VIRTFRDKTTEAVFDGESPKGFPADLVKVARRKLRYLHAAGELGDLRAPPGNRLEALSGNRKGQHSIRINDQFRVCFIWT
PQGPVEVEIVDYH

Nucleotide

• Length: 282 bp

>T36440 NZ_AP022639:c3832379-3832098 [Bradyrhizobium diazoefficiens]
GTGATCAGAACGTTTCGGGACAAAACGACTGAGGCAGTCTTCGACGGAGAAAGTCCCAAAGGCTTTCCAGCCGATCTCGT
GAAGGTTGCACGCCGGAAACTGCGTTATCTGCACGCTGCCGGCGAGCTCGGCGATCTAAGGGCGCCGCCCGGCAACCGTC
TGGAGGCCCTGAGCGGCAATCGCAAGGGACAGCACTCGATCAGGATCAATGATCAGTTCCGAGTGTGCTTCATCTGGACA
CCGCAAGGGCCCGTCGAGGTCGAGATTGTGGACTATCACTAG

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11266.07 Da
• Isoelectric Point: 9.0966

>AT36440 WP_011085795.1 NZ_AP022639:c3832086-3831778 [Bradyrhizobium diazoefficiens]
MAKKLKPMHPGEVLREEFLVPLNMSAGALAKTCGLPRTRIERIANEQTGITADTALRLAKALGTTAELWLNLQNDYDIQI
AKRDLGKALDRIETVNRPQAAE

Nucleotide

• Length: 309 bp

>AT36440 NZ_AP022639:c3832086-3831778 [Bradyrhizobium diazoefficiens]
ATGGCCAAAAAACTCAAGCCCATGCACCCCGGTGAGGTTCTACGGGAGGAGTTTCTAGTCCCACTGAACATGTCCGCTGG
CGCGCTCGCCAAGACGTGCGGCTTGCCGCGCACGCGCATCGAGCGGATCGCGAATGAGCAGACCGGAATCACCGCCGACA
CCGCGCTTCGACTGGCCAAGGCGCTCGGCACAACGGCCGAACTCTGGCTCAATCTTCAGAATGATTATGACATCCAGATC
GCGAAGCGCGATCTCGGCAAGGCACTTGACCGCATCGAGACCGTGAACAGGCCGCAAGCGGCTGAATGA

Structures

Toxin

• AlphaFold DB: Q89QW2