Detailed information of TA system TA036377

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 1123941..1124659
• Replicon: chromosome
• Accession: NZ_AP022615
• Organism: Mycobacterium heidelbergense strain JCM 14842

Toxin (Protein)

• Gene name: HigB2
• Uniprot ID: -
• Locus tag: G6N25_RS05265
• Protein ID: WP_083075119.1
• Coordinates: 1123941..1124354 (+)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: HigA2
• Uniprot ID: -
• Locus tag: G6N25_RS05270
• Protein ID: WP_083075077.1
• Coordinates: 1124351..1124659 (+)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6N25_RS05240	1120380..1120790	+	411	WP_083075087.1	DUF5319 domain-containing protein	-
G6N25_RS05245	1120818..1121615	-	798	WP_083075085.1	hypothetical protein	-
G6N25_RS05250	1121608..1122183	-	576	WP_083075083.1	sigma-70 family RNA polymerase sigma factor	-
G6N25_RS05255	1122265..1123053	-	789	WP_083075081.1	hypothetical protein	-
G6N25_RS05260	1123444..1123740	+	297	WP_083075079.1	WhiB family transcriptional regulator	-
G6N25_RS05265	1123941..1124354	+	414	WP_083075119.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
G6N25_RS05270	1124351..1124659	+	309	WP_083075077.1	XRE family transcriptional regulator	Antitoxin
G6N25_RS05275	1124648..1125862	-	1215	WP_083075075.1	cytochrome P450	-
G6N25_RS05280	1126026..1126487	-	462	WP_142272723.1	hypothetical protein	-
G6N25_RS05285	1127242..1127493	-	252	WP_083075072.1	type II toxin-antitoxin system RelE/ParE family toxin	-
G6N25_RS05290	1127490..1127762	-	273	WP_083075070.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
G6N25_RS05295	1127933..1128157	+	225	WP_083075068.1	hypothetical protein	-
G6N25_RS05300	1128148..1128558	+	411	WP_083075066.1	PIN domain nuclease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-109)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-87)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 15755.08 Da
• Isoelectric Point: 9.4062

>T36377 WP_083075119.1 NZ_AP022615:1123941-1124354 [Mycobacterium heidelbergense]
MRKWEVDLTLIEAWMDALDDDEYDNLIAALEQLEEHGPVTRRPFVDTIEGSKHPNMKELRPRATKAGAYIRVLFAFDIRS
RAIMLIAGDKAGNWSKWYAKHIPIADDLFTAHQKGLHREEAEATKAMNPKARKGKKR

Nucleotide

• Length: 414 bp

>T36377 NZ_AP022615:1123941-1124354 [Mycobacterium heidelbergense]
ATGCGGAAATGGGAGGTCGATCTGACCCTCATCGAAGCCTGGATGGACGCGCTCGACGACGACGAATACGACAACTTGAT
AGCAGCGCTGGAGCAGTTGGAGGAACACGGCCCAGTAACCCGGCGACCGTTCGTGGACACCATCGAGGGGTCAAAGCATC
CGAACATGAAAGAGCTTCGTCCGCGCGCCACGAAGGCCGGAGCCTACATTCGTGTGCTGTTCGCCTTCGATATCAGATCG
CGGGCAATCATGCTGATCGCTGGAGACAAGGCGGGGAATTGGTCCAAGTGGTATGCCAAGCACATCCCCATCGCCGACGA
CCTGTTCACTGCCCATCAGAAGGGTCTGCATAGGGAGGAAGCCGAAGCAACCAAAGCCATGAATCCGAAGGCAAGGAAGG
GGAAGAAACGATGA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11502.01 Da
• Isoelectric Point: 10.3882

>AT36377 WP_083075077.1 NZ_AP022615:1124351-1124659 [Mycobacterium heidelbergense]
MTNLDDMRRRRPGNRTRIDAIKAEMDREVTQYRLRELREAAGFTQTTLAAAIGVGQNRVSQMEHGELGTSRVDTLRRYVE
ATGGELEVSVKRPDGSRVLLSL

Nucleotide

• Length: 309 bp

>AT36377 NZ_AP022615:1124351-1124659 [Mycobacterium heidelbergense]
ATGACCAATCTCGACGATATGCGCCGTCGTCGCCCGGGCAACCGGACCCGTATTGACGCGATCAAGGCCGAGATGGACCG
CGAAGTCACCCAGTATCGTCTCCGTGAACTGCGCGAGGCCGCTGGCTTCACGCAGACGACCCTCGCTGCGGCTATTGGCG
TTGGCCAGAACCGTGTCTCCCAGATGGAGCACGGCGAGCTGGGCACTAGTCGTGTGGACACTCTCCGTAGGTACGTTGAG
GCGACGGGCGGTGAACTGGAGGTCTCCGTGAAGCGTCCGGACGGTTCACGGGTCCTCCTAAGTCTCTAG