Detailed information of TA system TA036298

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 3716723..3717399
• Replicon: chromosome
• Accession: NZ_AP022600
• Organism: Mycolicibacterium tokaiense strain JCM 6373

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: G6N58_RS18065
• Protein ID: WP_115277798.1
• Coordinates: 3717049..3717399 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: G6N58_RS18060
• Protein ID: WP_115277799.1
• Coordinates: 3716723..3717046 (-)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6N58_RS18050	3713736..3715133	+	1398	WP_115277800.1	FAD-binding oxidoreductase	-
G6N58_RS18060	3716723..3717046	-	324	WP_115277799.1	XRE family transcriptional regulator	Antitoxin
G6N58_RS18065	3717049..3717399	-	351	WP_115277798.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
G6N58_RS18070	3717435..3718781	-	1347	WP_115277797.1	site-specific integrase	-
G6N58_RS18075	3718778..3718963	-	186	WP_082967898.1	helix-turn-helix domain-containing protein	-
G6N58_RS18080	3719434..3722209	+	2776	Protein_3591	relaxase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (14-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-89)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13068.84 Da
• Isoelectric Point: 4.4346

>T36298 WP_115277798.1 NZ_AP022600:c3717399-3717049 [Mycolicibacterium tokaiense]
MAWVVLLLEEVERWYFTLDDDTMTSVTGAIDLLEAEGPALGRPTVDRVNGSTFHIMKELRPAGTSVRILFIFDPQRQAIL
LLGGDKAGNWRSWYDKNIPVADERYARWLATGEGGE

Nucleotide

• Length: 351 bp

>T36298 NZ_AP022600:c3717399-3717049 [Mycolicibacterium tokaiense]
ATGGCGTGGGTGGTGCTCCTGCTGGAGGAGGTTGAACGGTGGTACTTCACGCTGGACGACGACACCATGACGTCGGTGAC
CGGGGCGATCGACTTACTCGAGGCGGAGGGGCCCGCCTTGGGCCGGCCCACGGTGGACCGGGTGAACGGCTCGACGTTCC
ACATCATGAAGGAGCTGCGTCCCGCCGGTACCAGCGTCCGCATCCTGTTCATCTTCGATCCGCAGCGACAGGCGATCCTG
CTGCTCGGCGGAGACAAGGCCGGGAACTGGCGAAGCTGGTACGACAAGAACATCCCGGTCGCGGACGAGCGTTATGCCAG
ATGGCTGGCGACCGGCGAGGGAGGTGAGTAG

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 11759.20 Da
• Isoelectric Point: 6.9906

>AT36298 WP_115277799.1 NZ_AP022600:c3717046-3716723 [Mycolicibacterium tokaiense]
MARNWREIRAEAVEQGRVEPARADSARKQMHEAVQAYRLAEIRKAHGHARQADVAALMGVSQARVSQLESGDLAHTELRT
LQSYVAALGGHLRIVADFGEDSVELTA

Nucleotide

• Length: 324 bp

>AT36298 NZ_AP022600:c3717046-3716723 [Mycolicibacterium tokaiense]
ATGGCACGCAATTGGCGTGAGATCCGAGCCGAGGCGGTGGAACAGGGTCGCGTGGAACCCGCACGCGCAGACTCGGCACG
CAAGCAGATGCATGAAGCCGTCCAGGCTTACCGTCTCGCCGAGATTCGGAAGGCGCACGGCCACGCTCGCCAGGCCGACG
TCGCGGCGCTCATGGGCGTCTCGCAGGCGCGGGTCTCGCAATTGGAGAGCGGCGACTTGGCGCATACGGAGTTGAGGACG
CTGCAGTCCTACGTCGCCGCACTCGGTGGACACTTGCGGATTGTTGCCGACTTCGGTGAGGACAGCGTCGAACTGACGGC
CTGA