Detailed information of TA system TA036257

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 2860588..2861264
• Replicon: chromosome
• Accession: NZ_AP022586
• Organism: Mycolicibacterium litorale strain JCM 17423

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: G6N30_RS13575
• Protein ID: WP_134053586.1
• Coordinates: 2860588..2860938 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: G6N30_RS13580
• Protein ID: WP_134053588.1
• Coordinates: 2860941..2861264 (+)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6N30_RS13560	2855761..2858553	-	2793	WP_134055256.1	relaxase domain-containing protein	-
G6N30_RS13565	2859024..2859209	+	186	WP_197906176.1	helix-turn-helix domain-containing protein	-
G6N30_RS13570	2859206..2860552	+	1347	WP_134053584.1	site-specific integrase	-
G6N30_RS13575	2860588..2860938	+	351	WP_134053586.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
G6N30_RS13580	2860941..2861264	+	324	WP_134053588.1	XRE family transcriptional regulator	Antitoxin
G6N30_RS13585	2861302..2863068	-	1767	WP_163687567.1	AIPR family protein	-
G6N30_RS13595	2863874..2865262	-	1389	WP_134053592.1	FAD-binding oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	2851573..2863657	12084

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (14-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-89)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13152.88 Da
• Isoelectric Point: 4.4502

>T36257 WP_134053586.1 NZ_AP022586:2860588-2860938 [Mycolicibacterium litorale]
MAWVVLLLEEVERWYFTLDDDTMTAVTGAIDLLEAEGPALGRPTVDRVNGSKFHNMKELRPAGTSVRILFIFDPQRQAIL
LLGGDKARNWRSWYDENIPVADERYARWLATGDGGD

Nucleotide

• Length: 351 bp

>T36257 NZ_AP022586:2860588-2860938 [Mycolicibacterium litorale]
ATGGCGTGGGTAGTGCTCCTGCTGGAGGAGGTCGAGCGGTGGTACTTCACGCTGGACGACGACACGATGACGGCGGTGAC
CGGCGCCATCGACCTATTGGAGGCGGAGGGGCCCGCCTTGGGCCGGCCGACGGTGGACCGGGTGAACGGCTCGAAGTTCC
ACAACATGAAGGAGCTGCGTCCCGCCGGTACAAGTGTCCGCATCCTGTTCATCTTCGATCCGCAGCGCCAGGCGATCCTG
TTGCTCGGCGGAGACAAGGCCAGGAACTGGCGAAGCTGGTACGACGAGAACATCCCGGTCGCCGACGAGCGTTACGCCAG
ATGGCTGGCGACCGGTGACGGAGGTGATTAG

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 11564.92 Da
• Isoelectric Point: 6.6451

>AT36257 WP_134053588.1 NZ_AP022586:2860941-2861264 [Mycolicibacterium litorale]
MARNWRGIRAEAVEQGRVDSARADSARKEMHEAVQAYRLAEIRKAHGHARQADVAALMGVSQARVSQLESGDLAHTELGT
LQSYVAALGGHLRIVADFGEDSVELTA

Nucleotide

• Length: 324 bp

>AT36257 NZ_AP022586:2860941-2861264 [Mycolicibacterium litorale]
ATGGCGCGCAATTGGCGTGGAATTCGGGCCGAGGCGGTGGAACAGGGTCGCGTCGACTCAGCGCGTGCGGACTCGGCCCG
CAAGGAGATGCACGAAGCGGTCCAGGCGTACCGTCTCGCCGAGATCCGTAAGGCGCACGGTCACGCCCGTCAGGCCGACG
TCGCGGCACTCATGGGGGTCTCGCAGGCGCGGGTCTCGCAACTGGAGAGCGGCGACCTGGCGCATACGGAACTGGGCACG
CTGCAGTCTTACGTCGCCGCACTCGGCGGGCACTTGCGGATCGTTGCCGACTTCGGGGAGGACAGCGTCGAACTGACAGC
CTGA