Detailed information of TA system TA036124

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 19320..19562
• Replicon: plasmid pTHO-015-1
• Accession: NZ_AP022550
• Organism: Escherichia coli strain THO-015

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: JLF69_RS24550
• Protein ID: WP_001312861.1
• Coordinates: 19404..19562 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	19320..19360 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JLF69_RS24530	14515..16473	+	1959	WP_000117179.1	ParB/RepB/Spo0J family partition protein	-
JLF69_RS24535	16528..16962	+	435	WP_000845940.1	conjugation system SOS inhibitor PsiB	-
JLF69_RS24540	16959..17678	+	720	WP_001276232.1	plasmid SOS inhibition protein A	-
-	17690..17876	+	187	NuclAT_0	-	-
-	17690..17876	+	187	NuclAT_0	-	-
-	17690..17876	+	187	NuclAT_0	-	-
-	17690..17876	+	187	NuclAT_0	-	-
JLF69_RS24545	17924..19293	+	1370	WP_085947770.1	IS3-like element IS150 family transposase	-
-	19320..19360	+	41	NuclAT_1	-	Antitoxin
-	19320..19360	+	41	NuclAT_1	-	Antitoxin
-	19320..19360	+	41	NuclAT_1	-	Antitoxin
-	19320..19360	+	41	NuclAT_1	-	Antitoxin
JLF69_RS24550	19404..19562	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
JLF69_RS24555	20005..20340	+	336	WP_013023876.1	hypothetical protein	-
JLF69_RS24560	20253..20459	+	207	WP_000275859.1	hypothetical protein	-
JLF69_RS24565	20484..20771	+	288	WP_000107535.1	hypothetical protein	-
JLF69_RS24570	20890..21711	+	822	WP_001234469.1	DUF945 domain-containing protein	-
JLF69_RS24575	22008..22610	-	603	WP_000243712.1	transglycosylase SLT domain-containing protein	-
JLF69_RS24580	22941..23324	+	384	WP_001151566.1	relaxosome protein TraM	-
JLF69_RS24585	23458..24135	+	678	WP_001348626.1	PAS domain-containing protein	-
JLF69_RS24590	24223..24450	+	228	WP_001254388.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCTX-M-27 / tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / mph(A) / sul1 / qacE / aadA5	-	1..88121	88121

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T36124 WP_001312861.1 NZ_AP022550:19404-19562 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T36124 NZ_AP022550:19404-19562 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 41 bp

>AT36124 NZ_AP022550:19320-19360 [Escherichia coli]
TTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin