Detailed information of TA system TA036103

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 1921456..1921958
• Replicon: chromosome
• Accession: NZ_AP022549
• Organism: Escherichia coli strain THO-015

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: E2QNP2
• Locus tag: JLF69_RS09040
• Protein ID: WP_000767819.1
• Coordinates: 1921704..1921958 (+)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: E2QNP3
• Locus tag: JLF69_RS09035
• Protein ID: WP_001259253.1
• Coordinates: 1921456..1921707 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JLF69_RS09010	1916634..1917701	-	1068	WP_000080057.1	bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB	-
JLF69_RS09015	1917701..1918771	-	1071	WP_000108967.1	histidinol-phosphate transaminase	-
JLF69_RS09020	1918768..1920072	-	1305	WP_001296211.1	histidinol dehydrogenase	-
JLF69_RS09025	1920078..1920977	-	900	WP_000131782.1	ATP phosphoribosyltransferase	-
JLF69_RS09030	1921123..1921173	-	51	WP_001364200.1	his operon leader peptide	-
JLF69_RS09035	1921456..1921707	+	252	WP_001259253.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
JLF69_RS09040	1921704..1921958	+	255	WP_000767819.1	type II toxin-antitoxin system mRNA interferase toxin YoeB	Toxin
JLF69_RS09045	1922041..1922865	+	825	WP_000754737.1	SDR family oxidoreductase	-
JLF69_RS09050	1922911..1923840	+	930	WP_000803366.1	LysR family transcriptional regulator	-
JLF69_RS09055	1924055..1924117	+	63	WP_010723108.1	membrane protein YoeI	-
JLF69_RS09060	1924107..1925465	+	1359	WP_000019194.1	putrescine/proton symporter PlaP	-
JLF69_RS09065	1925682..1926140	+	459	WP_001531805.1	IS200/IS605-like element IS200C family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1925682..1926140	458

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (2-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-72)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10110.53 Da
• Isoelectric Point: 7.2749

>T36103 WP_000767819.1 NZ_AP022549:1921704-1921958 [Escherichia coli]
VKLIWSEESWDDYLYWQEADKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHLLVYAVTDDSLLIAAC
RYHY

Nucleotide

• Length: 255 bp

>T36103 NZ_AP022549:1921704-1921958 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAGCAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAACTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCTTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9365.62 Da
• Isoelectric Point: 4.6127

>AT36103 WP_001259253.1 NZ_AP022549:1921456-1921707 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSDKGTEKD
IIE

Nucleotide

• Length: 252 bp

>AT36103 NZ_AP022549:1921456-1921707 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGACAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Structures

Toxin

• AlphaFold DB: E2QNP2

Antitoxin

• AlphaFold DB: E2QNP3