Detailed information of TA system TA036094

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 105030..105294
• Replicon: plasmid pTHO-011-1
• Accession: NZ_AP022548
• Organism: Klebsiella michiganensis strain THO-011

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: E6BRV3
• Locus tag: JLG71_RS28305
• Protein ID: WP_001303307.1
• Coordinates: 105142..105294 (+)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	105030..105092 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JLG71_RS28290	100269..102560	-	2292	WP_024255997.1	hypothetical protein	-
JLG71_RS28295	102553..103623	-	1071	WP_000151583.1	IncI1-type conjugal transfer protein TrbB	-
JLG71_RS28300	103642..104850	-	1209	WP_000121273.1	IncI1-type conjugal transfer protein TrbA	-
-	105030..105092	-	63	NuclAT_0	-	Antitoxin
-	105030..105092	-	63	NuclAT_0	-	Antitoxin
-	105030..105092	-	63	NuclAT_0	-	Antitoxin
-	105030..105092	-	63	NuclAT_0	-	Antitoxin
JLG71_RS28305	105142..105294	+	153	WP_001303307.1	Hok/Gef family protein	Toxin
JLG71_RS28310	105366..105617	-	252	WP_001291965.1	hypothetical protein	-
-	106004..106055	-	52	NuclAT_1	-	-
-	106004..106055	-	52	NuclAT_1	-	-
-	106004..106055	-	52	NuclAT_1	-	-
-	106004..106055	-	52	NuclAT_1	-	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCTX-M-8	-	1..106439	106439

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5817.21 Da
• Isoelectric Point: 8.7948

>T36094 WP_001303307.1 NZ_AP022548:105142-105294 [Klebsiella michiganensis]
MPQRTFLMMLIVICVTILCFVWMVRDSLCVLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T36094 NZ_AP022548:105142-105294 [Klebsiella michiganensis]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCATCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGTACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin

Nucleotide

• Length: 63 bp

>AT36094 NZ_AP022548:c105092-105030 [Klebsiella michiganensis]
TCGAAGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: A0A634Q5L1

Antitoxin