Detailed information of TA system TA036070

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 86108..86534
• Replicon: plasmid pTHO-010-3
• Accession: NZ_AP022543
• Organism: Escherichia coli strain THO-010

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: JLH08_RS25555
• Protein ID: WP_001312861.1
• Coordinates: 86108..86266 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	86310..86534 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JLH08_RS25530	81482..82171	-	690	WP_000283385.1	conjugal transfer transcriptional regulator TraJ	-
JLH08_RS25535	82358..82741	-	384	WP_001151524.1	relaxosome protein TraM	-
JLH08_RS25540	83062..83664	+	603	WP_000243712.1	transglycosylase SLT domain-containing protein	-
JLH08_RS25545	83961..84782	-	822	WP_001234426.1	DUF945 domain-containing protein	-
JLH08_RS25550	84900..85187	-	288	WP_000107535.1	hypothetical protein	-
JLH08_RS25555	86108..86266	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	86310..86534	-	225	NuclAT_0	-	Antitoxin
-	86310..86534	-	225	NuclAT_0	-	Antitoxin
-	86310..86534	-	225	NuclAT_0	-	Antitoxin
-	86310..86534	-	225	NuclAT_0	-	Antitoxin
JLH08_RS25560	86346..86534	+	189	WP_001299721.1	hypothetical protein	-
JLH08_RS25565	86546..87265	-	720	WP_001276238.1	plasmid SOS inhibition protein A	-
JLH08_RS25570	87262..87696	-	435	WP_000845936.1	conjugation system SOS inhibitor PsiB	-
JLH08_RS25575	87751..87948	-	198	Protein_106	hypothetical protein	-
JLH08_RS25580	87976..88209	-	234	WP_000005987.1	DUF905 family protein	-
JLH08_RS25585	88277..88816	-	540	WP_000290840.1	single-stranded DNA-binding protein	-
JLH08_RS25590	88842..89048	-	207	WP_000275856.1	hypothetical protein	-
JLH08_RS25595	90187..91158	-	972	WP_000817028.1	ParB/RepB/Spo0J family plasmid partition protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aph(3')-Ia / blaCTX-M-55 / sitABCD	iutA / iucD / iucC / iucB / iucA	1..111439	111439

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T36070 WP_001312861.1 NZ_AP022543:c86266-86108 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T36070 NZ_AP022543:c86266-86108 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT36070 NZ_AP022543:c86534-86310 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin