Detailed information of TA system TA036066

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 63268..63522
• Replicon: plasmid pTHO-010-3
• Accession: NZ_AP022543
• Organism: Escherichia coli strain THO-010

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: JLH08_RS25385
• Protein ID: WP_001351576.1
• Coordinates: 63316..63522 (+)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	63268..63329 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JLH08_RS25360	60016..60762	+	747	WP_000205749.1	conjugal transfer pilus acetylase TraX	-
JLH08_RS25365	60821..61681	+	861	WP_000704513.1	alpha/beta hydrolase	-
JLH08_RS25370	61784..62344	+	561	WP_000139315.1	fertility inhibition protein FinO	-
JLH08_RS25375	62480..62692	+	213	WP_001299730.1	ANR family transcriptional regulator	-
JLH08_RS25380	62938..63012	+	75	Protein_67	endonuclease	-
-	63268..63329	-	62	NuclAT_1	-	Antitoxin
-	63268..63329	-	62	NuclAT_1	-	Antitoxin
-	63268..63329	-	62	NuclAT_1	-	Antitoxin
-	63268..63329	-	62	NuclAT_1	-	Antitoxin
JLH08_RS25385	63316..63522	+	207	WP_001351576.1	type I toxin-antitoxin system Hok family toxin	Toxin
JLH08_RS25390	63806..64063	+	258	WP_000083845.1	replication regulatory protein RepA	-
JLH08_RS25395	64295..64369	+	75	WP_001442103.1	RepA leader peptide Tap	-
JLH08_RS25400	64362..64844	+	483	WP_001273588.1	hypothetical protein	-
JLH08_RS25405	64837..65694	+	858	WP_000774297.1	incFII family plasmid replication initiator RepA	-
JLH08_RS25410	66395..66535	+	141	WP_001333237.1	hypothetical protein	-
JLH08_RS25415	66633..67286	+	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
JLH08_RS25420	67379..67636	+	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
JLH08_RS25425	67569..67970	+	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aph(3')-Ia / blaCTX-M-55 / sitABCD	iutA / iucD / iucC / iucB / iucA	1..111439	111439

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7826.31 Da
• Isoelectric Point: 8.8807

>T36066 WP_001351576.1 NZ_AP022543:63316-63522 [Escherichia coli]
MKYLNTTDCSLFFAERSKFMTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T36066 NZ_AP022543:63316-63522 [Escherichia coli]
ATGAAGTACCTTAACACTACTGATTGTAGCCTCTTCTTTGCTGAGAGGTCAAAGTTTATGACGAAATATGCCCTTATCGG
GTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTTATGTGAGCTGAATATTCACAGAG
GAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT36066 NZ_AP022543:c63329-63268 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin