Detailed information of TA system TA036062

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4740833..4741435
• Replicon: chromosome
• Accession: NZ_AP022540
• Organism: Escherichia coli strain THO-010

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: JLH08_RS22225
• Protein ID: WP_000897305.1
• Coordinates: 4741124..4741435 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JLH08_RS22220
• Protein ID: WP_000356397.1
• Coordinates: 4740833..4741123 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JLH08_RS22195	4736777..4737679	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
JLH08_RS22200	4737676..4738311	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
JLH08_RS22205	4738308..4739237	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
JLH08_RS22210	4739567..4739809	-	243	WP_001087409.1	hypothetical protein	-
JLH08_RS22215	4740029..4740247	-	219	WP_001315930.1	ribbon-helix-helix domain-containing protein	-
JLH08_RS22220	4740833..4741123	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
JLH08_RS22225	4741124..4741435	-	312	WP_000897305.1	hypothetical protein	Toxin
JLH08_RS22230	4741664..4742572	+	909	WP_001162704.1	alpha/beta hydrolase	-
JLH08_RS22235	4742636..4743577	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
JLH08_RS22240	4743622..4744059	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
JLH08_RS22245	4744056..4744928	-	873	WP_000920762.1	virulence factor BrkB family protein	-
JLH08_RS22250	4744922..4745521	-	600	WP_001295269.1	glucose-1-phosphatase	-
JLH08_RS22255	4745620..4746405	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T36062 WP_000897305.1 NZ_AP022540:c4741435-4741124 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T36062 NZ_AP022540:c4741435-4741124 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT36062 WP_000356397.1 NZ_AP022540:c4741123-4740833 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT36062 NZ_AP022540:c4741123-4740833 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7