Detailed information of TA system TA036041

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 636287..637086
• Replicon: chromosome
• Accession: NZ_AP022540
• Organism: Escherichia coli strain THO-010

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: A0A161R7C1
• Locus tag: JLH08_RS03010
• Protein ID: WP_000347278.1
• Coordinates: 636287..636751 (-)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: S1EB98
• Locus tag: JLH08_RS03015
• Protein ID: WP_001307405.1
• Coordinates: 636751..637086 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JLH08_RS02980	631288..631722	-	435	WP_000948824.1	PTS sugar transporter subunit IIA	-
JLH08_RS02985	631740..632618	-	879	WP_021557244.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
JLH08_RS02990	632608..633387	-	780	WP_000406214.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
JLH08_RS02995	633398..633871	-	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
JLH08_RS03000	633894..635174	-	1281	WP_021557243.1	tagatose-bisphosphate aldolase subunit KbaZ	-
JLH08_RS03005	635423..636232	+	810	WP_000072174.1	aga operon transcriptional regulator AgaR	-
JLH08_RS03010	636287..636751	-	465	WP_000347278.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
JLH08_RS03015	636751..637086	-	336	WP_001307405.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
JLH08_RS03020	637235..638806	-	1572	WP_001273763.1	galactarate dehydratase	-
JLH08_RS03025	639181..640515	+	1335	WP_000599633.1	galactarate/glucarate/glycerate transporter GarP	-
JLH08_RS03030	640531..641301	+	771	WP_001058227.1	2-dehydro-3-deoxyglucarate aldolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17866.28 Da
• Isoelectric Point: 9.6924

>T36041 WP_000347278.1 NZ_AP022540:c636751-636287 [Escherichia coli]
MDFPQRVNGWALYTHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Nucleotide

• Length: 465 bp

>T36041 NZ_AP022540:c636751-636287 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATACTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAGACAGAAGAAACCCATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12358.94 Da
• Isoelectric Point: 4.8616

>AT36041 WP_001307405.1 NZ_AP022540:c637086-636751 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT36041 NZ_AP022540:c637086-636751 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: A0A161R7C1

Antitoxin

• AlphaFold DB: A0A0E0XVC7