Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 620336..621135 Replicon chromosome
Accession NZ_AP022536
Organism Escherichia coli strain THO-008

Toxin (Protein)


Gene name yhaV Uniprot ID D3GWU5
Locus tag JLF34_RS02955 Protein ID WP_000347272.1
Coordinates 620336..620800 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag JLF34_RS02960 Protein ID WP_001307405.1
Coordinates 620800..621135 (-) Length 112 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JLF34_RS02925 615337..615771 - 435 WP_000948824.1 PTS sugar transporter subunit IIA -
JLF34_RS02930 615789..616667 - 879 WP_001295548.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
JLF34_RS02935 616657..617436 - 780 WP_000406214.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
JLF34_RS02940 617447..617920 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
JLF34_RS02945 617943..619223 - 1281 WP_000681955.1 tagatose-bisphosphate aldolase subunit KbaZ -
JLF34_RS02950 619472..620281 + 810 WP_000072171.1 aga operon transcriptional regulator AgaR -
JLF34_RS02955 620336..620800 - 465 WP_000347272.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
JLF34_RS02960 620800..621135 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
JLF34_RS02965 621284..622855 - 1572 WP_001273772.1 galactarate dehydratase -
JLF34_RS02970 623230..624564 + 1335 WP_000599651.1 galactarate/glucarate/glycerate transporter GarP -
JLF34_RS02975 624580..625350 + 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(12-150)

Antitoxin

(14-110)


Sequences


Toxin        


Download         Length: 155 a.a.        Molecular weight: 17832.26 Da        Isoelectric Point: 9.6924

>T36010 WP_000347272.1 NZ_AP022536:c620800-620336 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEEPH

Download         Length: 465 bp

>T36010 NZ_AP022536:c620800-620336 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAGACAGAAGAACCCCATTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT36010 WP_001307405.1 NZ_AP022536:c621135-620800 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT36010 NZ_AP022536:c621135-620800 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A829KUD2


Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References