Detailed information of TA system TA036000

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 32857..33099
• Replicon: plasmid pTHO-006-2
• Accession: NZ_AP022535
• Organism: Escherichia coli strain THO-006

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: JLF32_RS25110
• Protein ID: WP_001312861.1
• Coordinates: 32941..33099 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	32857..32897 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JLF32_RS25090	28052..30010	+	1959	WP_000117179.1	ParB/RepB/Spo0J family partition protein	-
JLF32_RS25095	30065..30499	+	435	WP_000845940.1	conjugation system SOS inhibitor PsiB	-
JLF32_RS25100	30496..31215	+	720	WP_001276232.1	plasmid SOS inhibition protein A	-
-	31227..31413	+	187	NuclAT_0	-	-
-	31227..31413	+	187	NuclAT_0	-	-
-	31227..31413	+	187	NuclAT_0	-	-
-	31227..31413	+	187	NuclAT_0	-	-
JLF32_RS25105	31461..32830	+	1370	WP_085947770.1	IS3-like element IS150 family transposase	-
-	32857..32897	+	41	NuclAT_1	-	Antitoxin
-	32857..32897	+	41	NuclAT_1	-	Antitoxin
-	32857..32897	+	41	NuclAT_1	-	Antitoxin
-	32857..32897	+	41	NuclAT_1	-	Antitoxin
JLF32_RS25110	32941..33099	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
JLF32_RS25115	33542..33877	+	336	WP_013023876.1	hypothetical protein	-
JLF32_RS25120	33790..33996	+	207	WP_000275859.1	hypothetical protein	-
JLF32_RS25125	34021..34308	+	288	WP_000107535.1	hypothetical protein	-
JLF32_RS25130	34427..35248	+	822	WP_001234469.1	DUF945 domain-containing protein	-
JLF32_RS25135	35545..36147	-	603	WP_000243712.1	transglycosylase SLT domain-containing protein	-
JLF32_RS25140	36478..36861	+	384	WP_001151566.1	relaxosome protein TraM	-
JLF32_RS25145	36994..37671	+	678	WP_001348626.1	PAS domain-containing protein	-
JLF32_RS25150	37759..37986	+	228	WP_001254388.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sul2 / aph(3'')-Ib / aph(6)-Id / tet(A) / blaCTX-M-27 / mph(A) / sul1 / qacE / aadA5 / dfrA17	-	1..101966	101966

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T36000 WP_001312861.1 NZ_AP022535:32941-33099 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T36000 NZ_AP022535:32941-33099 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 41 bp

>AT36000 NZ_AP022535:32857-32897 [Escherichia coli]
TTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin