Detailed information of TA system TA035998

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 110175..110428
• Replicon: plasmid pTHO-006-1
• Accession: NZ_AP022534
• Organism: Escherichia coli strain THO-006

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: A0A148HBD8
• Locus tag: JLF32_RS24910
• Protein ID: WP_001336447.1
• Coordinates: 110279..110428 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	110175..110231 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JLF32_RS24880	106087..106833	+	747	WP_113318688.1	conjugal transfer pilus acetylase TraX	-
JLF32_RS24885	106888..107445	+	558	WP_000139329.1	fertility inhibition protein FinO	-
JLF32_RS24890	107597..107800	+	204	WP_001336517.1	hypothetical protein	-
JLF32_RS24895	108542..109003	+	462	WP_019842128.1	thermonuclease family protein	-
JLF32_RS24900	109106..109490	+	385	Protein_143	hypothetical protein	-
JLF32_RS24905	109643..110080	+	438	WP_000872609.1	hypothetical protein	-
-	110175..110231	-	57	NuclAT_2	-	Antitoxin
-	110175..110231	-	57	NuclAT_2	-	Antitoxin
-	110175..110231	-	57	NuclAT_2	-	Antitoxin
-	110175..110231	-	57	NuclAT_2	-	Antitoxin
JLF32_RS24910	110279..110428	+	150	WP_001336447.1	type I toxin-antitoxin system Hok family toxin	Toxin
JLF32_RS24915	110713..110970	+	258	WP_000084404.1	replication regulatory protein RepA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..111171	111171

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5572.69 Da
• Isoelectric Point: 8.7678

>T35998 WP_001336447.1 NZ_AP022534:110279-110428 [Escherichia coli]
MTKYTLIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T35998 NZ_AP022534:110279-110428 [Escherichia coli]
ATGACGAAATATACCCTTATCGGGTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAGCTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 57 bp

>AT35998 NZ_AP022534:c110231-110175 [Escherichia coli]
GTACTTCATGCAGGCCTCACGAGTTAATGGATTAACAAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A148HBD8

Antitoxin