Detailed information of TA system TA035995

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 69460..69729
• Replicon: plasmid pTHO-006-1
• Accession: NZ_AP022534
• Organism: Escherichia coli strain THO-006

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: JLF32_RS24660
• Protein ID: WP_001312861.1
• Coordinates: 69571..69729 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	69460..69525 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JLF32_RS24630	65252..65783	+	532	Protein_89	single-stranded DNA-binding protein	-
JLF32_RS24635	65844..66075	+	232	Protein_90	DUF905 family protein	-
JLF32_RS24640	66140..68086	+	1947	Protein_91	ParB/RepB/Spo0J family partition protein	-
JLF32_RS24645	68141..68575	+	435	WP_000845923.1	conjugation system SOS inhibitor PsiB	-
JLF32_RS24650	68572..69291	+	720	WP_001276234.1	plasmid SOS inhibition protein A	-
JLF32_RS24655	69303..69491	-	189	WP_001336239.1	hypothetical protein	-
-	69303..69527	+	225	NuclAT_1	-	-
-	69303..69527	+	225	NuclAT_1	-	-
-	69303..69527	+	225	NuclAT_1	-	-
-	69303..69527	+	225	NuclAT_1	-	-
-	69460..69525	+	66	-	-	Antitoxin
JLF32_RS24660	69571..69729	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
JLF32_RS24665	70024..70179	+	156	WP_163034349.1	hypothetical protein	-
JLF32_RS24670	70177..70374	-	198	Protein_97	hypothetical protein	-
JLF32_RS24675	70376..70663	+	288	WP_000107535.1	hypothetical protein	-
JLF32_RS24680	70783..71604	+	822	WP_001234469.1	DUF945 domain-containing protein	-
JLF32_RS24685	71899..72540	-	642	WP_166687685.1	transglycosylase SLT domain-containing protein	-
JLF32_RS24690	72822..73205	+	384	WP_001151538.1	relaxosome protein TraM	-
JLF32_RS24695	73392..74081	+	690	WP_016239105.1	conjugal transfer transcriptional regulator TraJ	-
JLF32_RS24700	74180..74575	+	396	WP_001309237.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..111171	111171

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T35995 WP_001312861.1 NZ_AP022534:69571-69729 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T35995 NZ_AP022534:69571-69729 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 66 bp

>AT35995 NZ_AP022534:69460-69525 [Escherichia coli]
AGAAGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCAC

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin