Detailed information of TA system TA035990

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 25582..25852
• Replicon: plasmid pTHO-006-1
• Accession: NZ_AP022534
• Organism: Escherichia coli strain THO-006

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: JLF32_RS24360
• Protein ID: WP_001312861.1
• Coordinates: 25694..25852 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	25582..25645 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JLF32_RS24330	21351..21890	+	540	WP_001530505.1	single-stranded DNA-binding protein	-
JLF32_RS24335	21952..22185	+	234	WP_000005990.1	DUF905 family protein	-
JLF32_RS24340	22251..24209	+	1959	WP_200924004.1	ParB/RepB/Spo0J family partition protein	-
JLF32_RS24345	24264..24698	+	435	WP_000845923.1	conjugation system SOS inhibitor PsiB	-
JLF32_RS24350	24695..25414	+	720	WP_001276234.1	plasmid SOS inhibition protein A	-
JLF32_RS24355	25426..25614	-	189	WP_001336239.1	hypothetical protein	-
-	25426..25650	+	225	NuclAT_0	-	-
-	25426..25650	+	225	NuclAT_0	-	-
-	25426..25650	+	225	NuclAT_0	-	-
-	25426..25650	+	225	NuclAT_0	-	-
-	25582..25645	-	64	-	-	Antitoxin
JLF32_RS24360	25694..25852	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
JLF32_RS24365	26146..26301	+	156	WP_163034349.1	hypothetical protein	-
JLF32_RS24370	26299..26496	-	198	Protein_37	hypothetical protein	-
JLF32_RS24375	26498..26785	+	288	WP_000107535.1	hypothetical protein	-
JLF32_RS24380	26905..27726	+	822	WP_001234469.1	DUF945 domain-containing protein	-
JLF32_RS24385	28021..28662	-	642	WP_166687685.1	transglycosylase SLT domain-containing protein	-
JLF32_RS24390	28944..29327	+	384	WP_001151538.1	relaxosome protein TraM	-
JLF32_RS24395	29514..30203	+	690	WP_016239105.1	conjugal transfer transcriptional regulator TraJ	-
JLF32_RS24400	30302..30697	+	396	WP_001309237.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..111171	111171

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T35990 WP_001312861.1 NZ_AP022534:25694-25852 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T35990 NZ_AP022534:25694-25852 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 64 bp

>AT35990 NZ_AP022534:c25645-25582 [Escherichia coli]
GACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCTT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin