35968

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1919488..1919990 Replicon chromosome
Accession NZ_AP022533
Organism Escherichia coli strain THO-006

Toxin (Protein)


Gene name yoeB Uniprot ID S1P977
Locus tag JLF32_RS08985 Protein ID WP_000767822.1
Coordinates 1919736..1919990 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag JLF32_RS08980 Protein ID WP_001259255.1
Coordinates 1919488..1919739 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JLF32_RS08955 1914666..1915733 - 1068 WP_000080122.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
JLF32_RS08960 1915733..1916803 - 1071 WP_000108995.1 histidinol-phosphate transaminase -
JLF32_RS08965 1916800..1918104 - 1305 WP_001297912.1 histidinol dehydrogenase -
JLF32_RS08970 1918110..1919009 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
JLF32_RS08975 1919155..1919205 - 51 WP_001364200.1 his operon leader peptide -
JLF32_RS08980 1919488..1919739 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
JLF32_RS08985 1919736..1919990 + 255 WP_000767822.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
JLF32_RS08990 1920073..1920897 + 825 WP_000754733.1 SDR family oxidoreductase -
JLF32_RS08995 1920943..1921872 + 930 WP_000803366.1 LysR family transcriptional regulator -
JLF32_RS09000 1922087..1922149 + 63 WP_010723108.1 membrane protein YoeI -
JLF32_RS09005 1922139..1923497 + 1359 WP_000019194.1 putrescine/proton symporter PlaP -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T35968 WP_000767822.1 NZ_AP022533:1919736-1919990 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINEIIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T35968 NZ_AP022533:1919736-1919990 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAAATTATCAAAGATACACGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT35968 WP_001259255.1 NZ_AP022533:1919488-1919739 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT35968 NZ_AP022533:1919488-1919739 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1P977

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LNR0

References