Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1213222..1213949 Replicon chromosome
Accession NZ_AP022533
Organism Escherichia coli strain THO-006

Toxin (Protein)


Gene name higB Uniprot ID V0YLE2
Locus tag JLF32_RS05790 Protein ID WP_000547555.1
Coordinates 1213222..1213533 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A0H2V9Z2
Locus tag JLF32_RS05795 Protein ID WP_000126293.1
Coordinates 1213530..1213949 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JLF32_RS05760 1208363..1210072 + 1710 WP_001288134.1 formate hydrogenlyase subunit HycE -
JLF32_RS05765 1210082..1210624 + 543 WP_000493797.1 formate hydrogenlyase subunit HycF -
JLF32_RS05770 1210624..1211391 + 768 WP_000067399.1 formate hydrogenlyase subunit HycG -
JLF32_RS05775 1211388..1211798 + 411 WP_001291918.1 formate hydrogenlyase assembly protein HycH -
JLF32_RS05780 1211791..1212261 + 471 WP_000132956.1 hydrogenase maturation peptidase HycI -
JLF32_RS05785 1212304..1213059 + 756 WP_001305268.1 hypothetical protein -
JLF32_RS05790 1213222..1213533 + 312 WP_000547555.1 type II toxin-antitoxin system HigB family toxin Toxin
JLF32_RS05795 1213530..1213949 + 420 WP_000126293.1 helix-turn-helix domain-containing protein Antitoxin
JLF32_RS05800 1214041..1214469 - 429 WP_000536064.1 hypothetical protein -
JLF32_RS05805 1214854..1215381 + 528 WP_001078777.1 electron transport protein HydN -
JLF32_RS05810 1215534..1217786 + 2253 WP_001107659.1 carbamoyltransferase HypF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(22-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(85-138)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12439.18 Da        Isoelectric Point: 9.5146

>T35967 WP_000547555.1 NZ_AP022533:1213222-1213533 [Escherichia coli]
MHIISKAPFEECARKYPNDALALHSLYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Download         Length: 312 bp

>T35967 NZ_AP022533:1213222-1213533 [Escherichia coli]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTGTGCGCGCAAATATCCTAACGACGCTCTGGCTCTACACTCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACGCCAGAAGAAATGCGAACGGCTTTTCCCAACCTCGACAATTTTAAATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATCACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15426.46 Da        Isoelectric Point: 4.4596

>AT35967 WP_000126293.1 NZ_AP022533:1213530-1213949 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTIPHIKALSARFGVKPEWFL

Download         Length: 420 bp

>AT35967 NZ_AP022533:1213530-1213949 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAATATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCTGTT
GGTGTGGCGTTACTTCGCACGCTGATTGATCAACATAATCTGACCTATGCAGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGCCAGATCTTGTCTGGACAACGATCCTTGACGATACCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0YLE2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H2V9Z2

References