Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 355062..355708 Replicon chromosome
Accession NZ_AP022527
Organism Klebsiella pneumoniae strain THO-004

Toxin (Protein)


Gene name higB Uniprot ID W9BBY1
Locus tag JLF70_RS01645 Protein ID WP_016529833.1
Coordinates 355062..355409 (+) Length 116 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID W8UB68
Locus tag JLF70_RS01650 Protein ID WP_002920557.1
Coordinates 355409..355708 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JLF70_RS01635 350988..352421 + 1434 WP_023342584.1 glycogen synthase GlgA -
JLF70_RS01640 352439..354886 + 2448 WP_002920561.1 glycogen phosphorylase -
JLF70_RS01645 355062..355409 + 348 WP_016529833.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
JLF70_RS01650 355409..355708 + 300 WP_002920557.1 helix-turn-helix transcriptional regulator Antitoxin
JLF70_RS01655 355771..357279 - 1509 WP_023301456.1 glycerol-3-phosphate dehydrogenase -
JLF70_RS01660 357484..357813 + 330 WP_002920552.1 thiosulfate sulfurtransferase GlpE -
JLF70_RS01665 357864..358694 + 831 WP_024622927.1 rhomboid family intramembrane serine protease GlpG -
JLF70_RS01670 358744..359502 + 759 WP_002920548.1 DeoR/GlpR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(13-107)

Antitoxin

HigA2-like, helix-turn-helix domain

(9-87)


Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 13549.56 Da        Isoelectric Point: 5.6749

>T35945 WP_016529833.1 NZ_AP022527:355062-355409 [Klebsiella pneumoniae]
MWDVETTDTFDTWFELQSRALKEDMLATMLILSEFAPQLGRPYVDTVKDSTFQNMKELRVQHHGLPIRAFFAFDPLRKAI
VLCAGDKDGMNEKRFYKEMITLADREFSQHLTKER

Download         Length: 348 bp

>T35945 NZ_AP022527:355062-355409 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATACCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTTTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGCGGGCGATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10800.58 Da        Isoelectric Point: 10.1464

>AT35945 WP_002920557.1 NZ_AP022527:355409-355708 [Klebsiella pneumoniae]
MATLKELMAKQSPDSQQRIAAKAAEIRQSVALNLLREELQMSQTEMAAAMGVKQPTIAKMEQADNDPRLSTLKRYIAALG
GELSINVTLPTGKKVSFNL

Download         Length: 300 bp

>AT35945 NZ_AP022527:355409-355708 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGATAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCAGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB W9BBY1

Antitoxin

Source ID Structure
AlphaFold DB A0A0E1CBF8

References