Detailed information of TA system TA035940

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 80445..80678
• Replicon: plasmid pTHO-003-1
• Accession: NZ_AP022526
• Organism: Escherichia coli strain THO-003

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: JLF28_RS26090
• Protein ID: WP_001312861.1
• Coordinates: 80520..80678 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	80445..80476 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JLF28_RS26055	76566..77063	+	498	WP_042039995.1	single-stranded DNA-binding protein	-
JLF28_RS26060	77131..77364	+	234	WP_000005990.1	DUF905 family protein	-
JLF28_RS26065	77392..77589	+	198	Protein_87	hypothetical protein	-
JLF28_RS26070	77644..78078	+	435	WP_000845937.1	conjugation system SOS inhibitor PsiB	-
JLF28_RS26075	78075..78794	+	720	WP_001276238.1	plasmid SOS inhibition protein A	-
JLF28_RS26080	78806..78994	-	189	WP_001299721.1	hypothetical protein	-
-	78806..79003	+	198	NuclAT_0	-	-
-	78806..79003	+	198	NuclAT_0	-	-
-	78806..79003	+	198	NuclAT_0	-	-
-	78806..79003	+	198	NuclAT_0	-	-
JLF28_RS26085	79051..80420	+	1370	WP_085947770.1	IS3-like element IS150 family transposase	-
-	80445..80476	+	32	NuclAT_1	-	Antitoxin
-	80445..80476	+	32	NuclAT_1	-	Antitoxin
-	80445..80476	+	32	NuclAT_1	-	Antitoxin
-	80445..80476	+	32	NuclAT_1	-	Antitoxin
JLF28_RS26090	80520..80678	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
JLF28_RS26095	81011..82624	-	1614	WP_000080195.1	IS66-like element ISEc23 family transposase	-
JLF28_RS26100	82655..83005	-	351	WP_000624722.1	IS66 family insertion sequence element accessory protein TnpB	-
JLF28_RS26105	83002..83427	-	426	WP_000422741.1	IS66 family insertion sequence hypothetical protein	-
JLF28_RS26110	84139..84426	+	288	WP_000107535.1	hypothetical protein	-
JLF28_RS26115	84544..85365	+	822	WP_001234426.1	DUF945 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..123467	123467
	-	inside	IScluster/Tn	-	-	79051..83427	4376

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T35940 WP_001312861.1 NZ_AP022526:80520-80678 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T35940 NZ_AP022526:80520-80678 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 32 bp

>AT35940 NZ_AP022526:80445-80476 [Escherichia coli]
CACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin