Detailed information of TA system TA035937

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 5045379..5045981
• Replicon: chromosome
• Accession: NZ_AP022525
• Organism: Escherichia coli strain THO-003

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: JLF28_RS24625
• Protein ID: WP_000897302.1
• Coordinates: 5045670..5045981 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JLF28_RS24620
• Protein ID: WP_000356397.1
• Coordinates: 5045379..5045669 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JLF28_RS24590	5040686..5041615	+	930	WP_000027696.1	formate dehydrogenase accessory protein FdhE	-
JLF28_RS24595	5041797..5042039	-	243	WP_001068514.1	ribbon-helix-helix domain-containing protein	-
JLF28_RS24600	5042329..5043177	+	849	WP_001038650.1	hypothetical protein	-
JLF28_RS24605	5043493..5043942	+	450	WP_032140890.1	hypothetical protein	-
JLF28_RS24610	5044127..5044345	-	219	WP_001251293.1	ribbon-helix-helix domain-containing protein	-
JLF28_RS24615	5044742..5045020	-	279	WP_001296612.1	hypothetical protein	-
JLF28_RS24620	5045379..5045669	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
JLF28_RS24625	5045670..5045981	-	312	WP_000897302.1	hypothetical protein	Toxin
JLF28_RS24630	5046210..5047118	+	909	WP_001385591.1	alpha/beta hydrolase	-
JLF28_RS24635	5047182..5048123	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
JLF28_RS24640	5048168..5048605	-	438	WP_000560981.1	D-tyrosyl-tRNA(Tyr) deacylase	-
JLF28_RS24645	5048602..5049474	-	873	WP_000920762.1	virulence factor BrkB family protein	-
JLF28_RS24650	5049468..5050067	-	600	WP_001296610.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T35937 WP_000897302.1 NZ_AP022525:c5045981-5045670 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T35937 NZ_AP022525:c5045981-5045670 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT35937 WP_000356397.1 NZ_AP022525:c5045669-5045379 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT35937 NZ_AP022525:c5045669-5045379 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: S1P416