Detailed information of TA system TA035916

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 1932620..1933122
• Replicon: chromosome
• Accession: NZ_AP022525
• Organism: Escherichia coli strain THO-003

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: E2QNP2
• Locus tag: JLF28_RS09100
• Protein ID: WP_000767819.1
• Coordinates: 1932868..1933122 (+)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: E2QNP3
• Locus tag: JLF28_RS09095
• Protein ID: WP_001259253.1
• Coordinates: 1932620..1932871 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JLF28_RS09070	1927798..1928865	-	1068	WP_000080057.1	bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB	-
JLF28_RS09075	1928865..1929935	-	1071	WP_000108967.1	histidinol-phosphate transaminase	-
JLF28_RS09080	1929932..1931236	-	1305	WP_001296211.1	histidinol dehydrogenase	-
JLF28_RS09085	1931242..1932141	-	900	WP_000131782.1	ATP phosphoribosyltransferase	-
JLF28_RS09090	1932287..1932337	-	51	WP_001364200.1	his operon leader peptide	-
JLF28_RS09095	1932620..1932871	+	252	WP_001259253.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
JLF28_RS09100	1932868..1933122	+	255	WP_000767819.1	type II toxin-antitoxin system mRNA interferase toxin YoeB	Toxin
JLF28_RS09105	1933205..1934029	+	825	WP_000754737.1	SDR family oxidoreductase	-
JLF28_RS09110	1934075..1935004	+	930	WP_000803366.1	LysR family transcriptional regulator	-
JLF28_RS09115	1935219..1935281	+	63	WP_010723108.1	membrane protein YoeI	-
JLF28_RS09120	1935271..1936629	+	1359	WP_000019194.1	putrescine/proton symporter PlaP	-
JLF28_RS09125	1936846..1937304	+	459	WP_001531805.1	IS200/IS605-like element IS200C family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1936846..1937304	458

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (2-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-72)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10110.53 Da
• Isoelectric Point: 7.2749

>T35916 WP_000767819.1 NZ_AP022525:1932868-1933122 [Escherichia coli]
VKLIWSEESWDDYLYWQEADKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHLLVYAVTDDSLLIAAC
RYHY

Nucleotide

• Length: 255 bp

>T35916 NZ_AP022525:1932868-1933122 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAGCAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAACTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCTTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9365.62 Da
• Isoelectric Point: 4.6127

>AT35916 WP_001259253.1 NZ_AP022525:1932620-1932871 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSDKGTEKD
IIE

Nucleotide

• Length: 252 bp

>AT35916 NZ_AP022525:1932620-1932871 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGACAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Structures

Toxin

• AlphaFold DB: E2QNP2

Antitoxin

• AlphaFold DB: E2QNP3