Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3820484..3821141 Replicon chromosome
Accession NZ_AP022519
Organism Enterobacter cloacae strain STN0717-73

Toxin (Protein)


Gene name higB Uniprot ID A0A808IF03
Locus tag JLF40_RS18440 Protein ID WP_033146338.1
Coordinates 3820484..3820783 (+) Length 100 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A808ICP3
Locus tag JLF40_RS18445 Protein ID WP_033146339.1
Coordinates 3820794..3821141 (+) Length 116 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JLF40_RS18430 3816852..3819050 + 2199 WP_033146337.1 type I secretion system permease/ATPase -
JLF40_RS18435 3819061..3820230 + 1170 WP_086374946.1 HlyD family efflux transporter periplasmic adaptor subunit -
JLF40_RS18440 3820484..3820783 + 300 WP_033146338.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
JLF40_RS18445 3820794..3821141 + 348 WP_033146339.1 HigA family addiction module antidote protein Antitoxin
JLF40_RS18450 3821186..3821611 + 426 WP_033146340.1 nucleoside triphosphatase NudI -
JLF40_RS18455 3821583..3822314 - 732 WP_033146341.1 helix-turn-helix transcriptional regulator -
JLF40_RS18460 3822412..3822831 + 420 WP_033146342.1 nuclear transport factor 2 family protein -
JLF40_RS18465 3822834..3823352 - 519 WP_033146343.1 hypothetical protein -
JLF40_RS18470 3823437..3824618 - 1182 WP_033146344.1 PLP-dependent aminotransferase family protein -
JLF40_RS18475 3824638..3825525 - 888 WP_033146345.1 LysR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(1-95)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(8-86)


Sequences

Toxin        


Download         Length: 100 a.a.        Molecular weight: 11691.32 Da        Isoelectric Point: 10.0346

>T35905 WP_033146338.1 NZ_AP022519:3820484-3820783 [Enterobacter cloacae]
MISYFRDQWLEDFFLYGRSSNVIPANLETALARKLDIIRAATSHRDLRSPPGNMYEALNPPLKGYSSIRVNRQYRLVFRW
TEGKAEDLYLSPHKYTQHK

Download         Length: 300 bp

>T35905 NZ_AP022519:3820484-3820783 [Enterobacter cloacae]
ATGATTAGTTATTTTCGGGATCAATGGCTTGAGGATTTTTTTCTTTACGGCAGATCGAGCAATGTTATTCCTGCAAATCT
GGAAACAGCGCTTGCGAGAAAGCTCGATATCATCAGGGCAGCAACCTCGCACAGGGATTTGCGATCGCCACCGGGCAATA
TGTATGAAGCATTGAATCCTCCGCTGAAGGGATATTCCTCAATCCGGGTAAACAGGCAATACAGGCTCGTATTTCGCTGG
ACAGAGGGTAAAGCAGAAGATCTCTACCTCTCTCCACACAAGTACACGCAACACAAGTGA

Antitoxin


Download         Length: 116 a.a.        Molecular weight: 13066.02 Da        Isoelectric Point: 7.0134

>AT35905 WP_033146339.1 NZ_AP022519:3820794-3821141 [Enterobacter cloacae]
MTLQQALRKPTTPGDVLQYEYLEPLNLKISDLAEMLNVHRNTISALVNNNRKLTADMAIKLAKAFDTTIEFWLNLQLNVD
IWEAQSNPRTQEELSRIKTVADVMAKRKSGQPDVA

Download         Length: 348 bp

>AT35905 NZ_AP022519:3820794-3821141 [Enterobacter cloacae]
ATGACACTTCAACAGGCACTCCGCAAACCCACCACGCCGGGCGACGTGTTGCAGTATGAGTATCTTGAACCGCTCAATCT
GAAAATCAGCGATCTGGCGGAGATGCTCAATGTTCACCGCAACACCATCAGCGCGCTGGTCAATAATAATCGCAAGCTGA
CTGCCGATATGGCGATCAAACTGGCAAAAGCCTTTGATACCACGATTGAATTTTGGCTTAACTTACAGCTGAACGTCGAT
ATCTGGGAAGCGCAATCCAACCCCAGGACGCAGGAGGAGCTAAGCCGTATAAAGACCGTTGCGGACGTCATGGCTAAGCG
AAAATCTGGCCAGCCGGATGTAGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A808IF03

Antitoxin

Source ID Structure
AlphaFold DB A0A808ICP3

References